长链非编码 RNA AFAP1－AS1在乳腺癌中的表达及临床意义

目的：探讨长链非编码 RNA（long non －coding RNA，lncRNA）肌动蛋白纤维相关蛋白1－反义 RNA1（actinfilament －associated protein 1－antisense RNA1，AFAP1－AS1）在乳腺癌肿瘤组织中的表达情况及其临床意义。方法：采用荧光定量 PCR 方法检测76例乳腺癌组织及其对应癌旁组织中 AFAP1－AS1的表达情况。选取表达差异最大的5对组织，结合核浆分离的方法，检测 AFAP1－AS1在乳腺癌细胞中的表达位置。分析AFAP1－AS1表达与乳腺癌主要临床病理特征及患者生存曲线的相关性。通过 siRNA 干扰 AFAP1－AS1在乳腺癌细胞 SKBR －3、MCF －7中的表达，MTT 法检测其表达对乳腺癌细胞增殖的影响。结果：AFAP1－AS1在乳腺癌组织中的表达水平相较癌旁组织明显下调（P ＜0．05），并且这一表达差异主要发生在细胞质。沉默AFAP1－AS1的表达会促进乳腺癌细胞增殖。同时，AFAP1－AS1在乳腺癌组织中的表达差异与临床分期和淋巴结转移有关（P ＜0．05），且与患者生存率也明显相关（P ＜0．05）。结论：lncRNA AFAP1－AS1在乳腺癌组织中表达下调，影响乳腺癌细胞增殖，可能与乳腺癌的发生和发展有关，可能成为新的乳腺癌分子标志物。

The expression and clinical significance of a long non -coding RNA(lncRNA),actin fila-ment -associated protein 1 antisense RNA 1(AFAP1 -AS1)in breast cancer

Objective:To investigate the expression and clinical significance of actin filament -associated protein 1 antisense RNA 1(AFAP1 -AS1),a long non -coding RNA(lncRNA)in breast cancer.Methods:The expression of AFAP1 -AS1 was detected in 76 cases of breast tumor tissues and their corresponding adjacent tissues by Real -time fluorescent quantitative PCR(qRT -PCR).Combined with nucleoplasm separation,five pairs of tissues in which the expression differences were significant were chosen to detect the location of AFAP1 -AS1 in tumor cells by qRT -PCR.The relationship between the expression of AFAP1 -AS1 and the clinicopathological features was analyzed. AFAP1 -AS1 -siRNA was designed according to AFAP1 -AS1 gene sequence and transfected into SKBR -3 and MCF -7 cells.The proliferation of SKBR -3 and MCF -7 cells were analyzed by MTT.Results:The expression of AFAP1 -AS1 in breast cancer tissues was significantly lower than that in their corresponding adjacent normal tissues (P <0.05),and AFAP1 -AS1 was expressed in cytoplasm.Silence of AFAP1 -AS1 increased the proliferation of SKBR -3 and MCF -7 cells.The expression difference of AFAP1 -AS1 in breast cancer tissues was associated with clinical stage and lymph node metastasis(P <0.05).This difference affected the survival rates of breast cancer pa-tients as well.Conclusion:The expression of AFAP1 -AS1 downregulated in breast cancer tissues and was associated with cell proliferation.It is suggested that AFAP1 -AS1 may play a role in the pathogenesis and progression of breast cancer and become a noval molecular marker for clinical diagnosis.