两种乳腺癌相关基因联合检测的临床应用

目的:建立逆转录聚合酶链反应(RT-PCR)法检测乳腺癌人类表皮生长因子受体2(human epidermal growth factor receptor-2，C-erbB-2)和人乳腺珠蛋白（human mammaglobin，HMAM）基因表达水平，探讨其联合检测在乳腺癌诊断中的应用。方法:采用RT-PCR法，以β-actin为内对照，测定40例健康女性体检者、69例良性乳腺肿瘤和127例乳腺癌外周血中C-erbB-2和HMAM的表达量。结果:外周血中C-erbB-2和HMAM在良性乳腺疾病组和健康体检组的表达率无差异，乳腺癌组和良性乳腺疾病组以及健康体检组的表达率有显著性差异。HMAM和C-erbB-2联合检测的灵敏度显著高于单一基因。结论:RT-PCR联合检测外周血HMAM和C-erbB-2基因表达，可提高对乳腺癌诊断的灵敏度，有效辅助临床诊断乳腺癌。

Study of combined detection of two genes express in the diagnosis of breast cancer

Objective:To evaluate combined detection of C -erbB -2 and HMAMgene in the diagnosis of breast cancer.Methods:We used RT -PCR to detect C -erbB -2 and HMAM mRNA express in clinical samples from 40 health women and 127 patients with breast cancer and 69 patients with benign breast disease,β2 -microglobin was used as internal control.Results:No significant difference was found in the positive rate of C -erbB -2 and HMAM between normal controls and benign breast disease group(P >0.05).The positive rate of C -erbB -2 and HMAMin breast cancer group were higher than those in normal controls and benign breast disease group(P <0.05).The speci-ficity of combined detection of C -erbB -2 and HMAMgene express was higher than that of single gene detection(P<0.05).Conclusion:Combined detection of C -erbB -2 and HMAMin peripheral blood by RT -PCR can improve the specificity of diagnosis of breast cancer,it also can effectively assist the diagnosis of breast cancer.