Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies |
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Authors: | J M Wilkinson D L Wetterskog J A Sogn T J Kindt |
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Affiliation: | Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20205, U.S.A. |
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Abstract: | The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000 mol. wt molecule is similar or identical to a rabbit class I major histocompatibility complex antigen and its characterization has been reported elsewhere. Three different 90,000 mol. wt proteins can be distinguished by their reactivity with lectins and by sequential immunoprecipitation. The 120,000 mol. wt protein is a very abundant surface glycoprotein that appears to be a specific marker for T-cells in the rabbit. It is the immunodominant antigen in a lentil lectin bound glycoprotein pool. Over half of the antibodies were directed against this antigen. All antigens detected by the panel of monoclonal antibodies have been detected on normal lymphoid cells. |
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Keywords: | FCS fetal calf serum LcH MHC major histocompatibility complex RCA SDS sodium dodecyl sulfate HBSS Hanks' balanced salt solution WGA wheat germ agglutinin NP-40 Nonidet P-40 BSA bovine serum albumin PBS ELISA enzyme-linked immunosorbent assay RIA radioimmunoassay |
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