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Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen |
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| Authors: | Irina Sominskaya Wilma Paulij Juris Jansons Dirk Sobotta Dzidra Dreilina Cecile Sunnen Helga Meisel Wolfram H. Gerlich Paul Pumpens |
| Affiliation: | a Biomedical Research and Study Centre, University of Latvia, Ratsupites Str. 1, Riga, LV-1067, Latvia b Biosciences Research Unit, Organon Teknika BV, Boxtel, The Netherlands c Institute of Medical Virology, Justus-Liebig-University, Giessen, Germany d Institute of Virology, Humboldt University, Charité Medical School, Berlin, Germany |
| Abstract: | In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants. |
| Keywords: | Hepatitis B virus preS2 domain Monoclonal antibody Epitope mapping |
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