| Interleukin-15 Promotes the Commitment of Cord Blood CD34^＋ Stem Cells into NK Cells |
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| 引用本文： | 张建,夏青,孙汭,田志刚.Interleukin-15 Promotes the Commitment of Cord Blood CD34^＋ Stem Cells into NK Cells[J].中华微生物学和免疫学杂志(英文版),2004,2(1):40-44. |
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| 作者姓名： | 张建 夏青 孙汭 田志刚 |
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| 作者单位： | Institute of Basic Medicine，Shandong Academy of Medical Science，Institute of Basic Medicine，Shandong Academy of Medical Science，Institute of Basic Medicine，Shandong Academy of Medical Science，Institute of Basic Medicine，Shandong Academy of Medical Science Jinan 250062，China，Jinan 250062，China，Jinan 250062，China，Jinan 250062，China |
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| 基金项目： | ThisprojectwassupportedbygrantsfromtheNationalNaturalSciencFoundationofChina(No.39870729,39970828)andShangdongNaturalScienceFoundation(No.Z2000C01) |
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| 摘 要： | Naturalkiller(NK)cellsweresubgroupoflymphocytes thatplayedanessentialroleinthecellularbasedimmune defenseagainstvirus infectedandtransfectedcells.His torically,inteleukin 2(IL 2)wasregardedasthenatural activatorforNKcells.Recently,itwasdiscoveredthat IL …
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| 关 键 词： | 白细胞间介素-15 增进剂 CD34^＋ 造血干细胞 NK细胞 MACS 基因表达 脐血 |
Interleukin-15 Promotes the Commitment of Cord Blood CD34~ Stem Cells into NK Cells |
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| Jian ZHANG Qing XIA Rui SUN and Zhigang TIAN Institute of Basic Medicine,Shandong Academy of Medical Science,Jinan ,China.Interleukin-15 Promotes the Commitment of Cord Blood CD34~ Stem Cells into NK Cells[J].Chinese Journal of Microbiology and Immunology,2004,2(1):40-44. |
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| Authors: | Jian ZHANG Qing XIA Rui SUN and Zhigang TIAN Institute of Basic Medicine Shandong Academy of Medical Science Jinan China |
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| Institution: | InstituteofBasicMedicine,ShandongAcademyofMedicalScience,Jinan250062,China. |
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| Abstract: | To explore the effect of rhIL-15 on CB-CD34 stem cells committing to NK cells, CD34 stem cells were obtained from cord blood (CB) by magnetic-assisted cell sorting (MACS) method. CD3, CD16 and CD56 molecules expressed on cell surface were detected by flow cytometer. MTT method was used to test the cytotoxicity of NK cells. The results were that stem cell factor (SCF) alone has no effect on CD34 stem cells. IL-15 stimulated CD34 stem cells commit to NK cells, and SCF showed strong synergistic effect with IL-15. It was concluded that IL-15 and SCF played different roles during NK cell development, IL-15 promoted CD34 stem cells differentiate to NK cell precursor and SCF improved the effects of IL-15 on NK cell differentiation. |
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| Keywords: | IL-15 Differentiation NK cell Stem cell |
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