| 麦考酚酸产生菌原生质体诱变育种的研究 |
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| 引用本文： | 杨亚勇,李长洪,孟文伟.麦考酚酸产生菌原生质体诱变育种的研究[J].中国抗生素杂志,2006,31(10):587-590. |
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| 作者姓名： | 杨亚勇 李长洪 孟文伟 |
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| 作者单位： | 广东新北江制药股份有限公司,清远,511515 |
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| 摘 要： | 对麦考酚酸产生菌（Penicillium brevicomactum）的原生质体制备、再生条件进行了研究，建立了原生质体诱变筛选体系。在此基础上，采用紫外线和亚硝酸复合诱变处理麦考酚酸产生菌的原生质体，经原生质体再生获得了A-S-8的菌株，该菌株比对照菌株生产能力提高28．2％，摇瓶和50L发酵实验的效价均可达到6000mg／L以上。
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| 关 键 词： | 麦考酚酸 原生质体 诱变 |
| 文章编号： | 1001-8689(2006)10-0587-04 |
| 收稿时间： | 2006-04-13 |
| 修稿时间： | 2006-05-08 |
Protoplast mutation breeding of mycophenolic acid producing strain, Penicillium brevicompactum |
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| Yang Ya-yong,Li Chang-hong,Meng Wen-wei.Protoplast mutation breeding of mycophenolic acid producing strain, Penicillium brevicompactum[J].Chinese Journal of Antibiotics,2006,31(10):587-590. |
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| Authors: | Yang Ya-yong Li Chang-hong Meng Wen-wei |
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| Institution: | Guangdong New North River Pharmaceutical Co. Ltd., Qingyuan 511515 |
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| Abstract: | To select high toplast preparation, regeneration and mycophenolic acid producing mutants from mutagenesis were carried out. The mutant Penicillium brevicompactum, the prostrain A-S-8 of Penicillium brevicompactum which possessed high yield of mycophenolic acid was obtained through combined induction with UV and HNO2. The mycophenolic acid yield of mutant was increased by 28.2% compared with the starting strain. The mycophenolic acid titre of strain A-S-8 reached above 6000mg/L both in shaking flask and 50-liter fermenter experiments. |
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| Keywords: | Mycophenolic acid Protoplast Mutation |
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