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Axonal elongation into peripheral nerve grafts between thalamus and somatosensory cortex of the rat. An experimental model
Authors:M. Cossu   A. Martelli   A. Pau   E. Sehrbundt Viale   D. Siccardi  G.L. Viale
Affiliation:1. Department of Neuroscience, Brown University, 185 Meeting Street, Box G-LN, Providence, RI 02912, USA;1. CNRS, INCIA, UMR 5287, Bordeaux, France;2. University of Bordeaux, INCIA, UMR 5287, Bordeaux, France;3. Institute of Physiology and Pathophysiology, Medical Biophysics, Heidelberg University, INF 326, 69120, Heidelberg, Germany;1. Blue Brain Project, École polytechnique fédérale de Lausanne (EPFL), Campus Biotech, Geneva, Switzerland;2. Laboratory of Neural Microcircuitry (LNMC), École polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland;3. Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain;4. Department of Neurobiology, Hebrew University of Jerusalem, Jerusalem, Israel;5. Department of Psychiatry, University of Toronto, Toronto, Canada;6. Department of Physiology, University of Toronto, Toronto, Canada;7. Krembil Centre for Neuroinformatics, Centre for Addiction and Mental Health (CAMH), Toronto, Canada
Abstract:After destruction of the thalamocortical projections of one side in 12 adult rats, the ipsilateral thalamic ventrobasal nucleus was connected with the contralateral somatosensory cortex through an autologous peripheral nerve graft. Three months after the implanting procedure, horseradish peroxidase was applied to the transected nerve. Retrograde labeling of cells occurred in all examined animals. Evidence of newly formed thalamocortical connections provides the experimental basis to a model suitable for functional evaluations.
Keywords:Nerve graft   Axonal elongation   Thalamocortical connection   Somatosensory pathway   Tracing technique   Horseradish peroxidase
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