Electron microscopic evidence for scorpion toxin binding to synapses of rat brain cortex |
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| Authors: | Alfonso Carabez Trejo Lourival Domingos Possani |
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| Affiliation: | 1. Sección de Microscopía Electronica Centro de Investigación en Fisiologia Celular, UNAM, Apartado Postal 70-600 Mexico;1. Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, UNAM, Apartado Posttal 70-247, Delegación Coyoacán 04510, México, D.F. Mexico |
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| Abstract: | The venom from the scorpion Centruroides noxius Hoffman was fractionated by Sephadex G-50 gel filtration. The toxic fraction (n.II) was coupled to Sepharose-4B and used for purification of specific horse immunoglobulins. The purified immunoglobulins were linked to ferritin with glutaraldehyde and repurified in the same affinity column. This complex bound to the postsynaptic membrane of rat brain tissue previously exposed to toxin. |
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| Keywords: | To whom correspondence should be addressed. |
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