氧化型低密度脂蛋白对骨髓内皮前体细胞增殖和细胞功能及凋亡的影响

目的研究氧化型低密度脂蛋白(ox-LDL)对骨髓内皮前体细胞(EPCs)增殖抑制、细胞功能及促凋亡的影响,观察阿托伐他汀是否对其有保护作用。方法密度梯度离心法从猪的骨髓中分离出EPCs,将细胞分为3组,A组(培养液中不加ox-LDL),B组(ox-LDL 10 mg/L),C组(预先给予阿托伐他汀1μmol/L+ox-LDL 10 mg/L)。四甲基偶氮唑盐法检测细胞的增殖能力、流式细胞仪检测细胞凋亡,同时测定细胞培养液中一氧化氮及一氧化氮合酶含量,观察对细胞功能的影响。结果ox-LDL可引起骨髓EPCs增殖能力降低,促进细胞凋亡,使细胞功能受损,阿托伐他汀可以减弱ox-LDL对细胞的不良影响。结论阿托伐他汀可以减弱ox-LDL引起的EPCs增殖能力降低,对ox-LDL引起的EPC凋亡及细胞功能的损害有保护作用。

Effect of oxidized low-density lipoprotein on proliferation, apoptosis and cell function of endothelial progenitor cells of bone marrow

Objective To study the effect of oxidized low-density lipoprotein(ox-LDL) on the proliferation,apoptosis and cell function of endothelial progenitor cells(EPCs),and to observe whether atorvastatin has protective effect against the changes induced by ox-LDL.Methods Bone marrow derived EPCs were isolated from bone-marrow of swine by density gradient centrifugation.The cells were divided into three groups.There was no ox-LDL in the culture medium of group A, ox-LDL(10 mg/L) was added to group B,atorvastatin(1 μmol/L) and ox-LDL(10 mg/L) were added to culture medium of group C.MTT assay was used to detect the proliferation ability of EPCs and flow cytometry was used to detect the apoptosis of cells.NO and NOS(cNOS,iNOS) contents in the cell culture medium were measured to observe the effect of ox-LDL on the cell functions.Results ox-LDL could decrease the proliferation ability of EPCs,promote the apoptosis of cells and damage the cells' function.These harmful effects could be attenuated by atorvastatin.Conclusion Atorvastatin has protective effect against the decrease in cell proliferation ability,apoptosis promotion and damage of cell function induced by ox-LDL.