| 膦甲酸钠氯化钠注射液有关物质和含量的测定 |
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| 引用本文： | 江海峰,邓岩辉,孟祥军.膦甲酸钠氯化钠注射液有关物质和含量的测定[J].中国药业,2009,18(9):32-33. |
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| 作者姓名： | 江海峰 邓岩辉 孟祥军 |
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| 作者单位： | 沈阳医学院,辽宁,沈阳,110034 |
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| 摘 要： | 目的建立测定膦甲酸钠氯化钠注射液有关物质和含量的方法。方法采用高效液相色谱（HPLC）法测定有关物质，固定相为聚合阴离子树脂（Waters IC PAK，5cm×4．6mm），流动相为邻苯二甲酸氢钾-1mol／L硝酸溶液，检测波长为290nm，流速为1．4mL／min，柱温为25℃；采用紫外分光光度（UV）法测定膦甲酸钠注射液的含量。结果膦甲酸钠质量浓度在0．24～0．36mg／mL时与吸收度具有良好的线性关系（r=0．9998，n=5），平均回收率为100．06％，RSD=0．95％（n=9）。结论所用方法简便、快速、准确，可用于膦甲酸钠氯化钠注射液有关物质和含量的测定。
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| 关 键 词： | 膦甲酸钠 高效液相色谱法 有关物质 紫外分光光度法 |
Determination of Foscarnel Sodium and Its Related Substances |
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| Gang Haifeng,Oeng Yanhui,Meng Xiangjun.Determination of Foscarnel Sodium and Its Related Substances[J].China Pharmaceuticals,2009,18(9):32-33. |
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| Authors: | Gang Haifeng Oeng Yanhui Meng Xiangjun |
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| Institution: | ( Shenyang Medical College, Shenyang, Liaoning, China 110034) |
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| Abstract: | Objective To establish a method for the assay of foscarnel sodium and its related substances by HPLC. Methods The aggregating anion colophony column（Waters IC PAK,5 cm×4.6 mm）was used. The mobile phase was potassium hydrogen phthalate-nitric acid solution （1 mol/L）. The detection wavelength was set at 290 nm. The flow rate was 1.4 mL/min. The column temperature was 25 ℃. Results The linear range of foscarnel sodium was 0. 24-0.36 mg/mL （r =0. 999 8, n =5）. The average recovery rate was 100. 06%, RSD =0.95% （n =9）. Conclusion The method is simple, rapid and accurate. It can be used to determine the content of foscarnel sodium and its related substances in Foscarnel Sodium Injection. |
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| Keywords: | foscarnel sodium HPLC related substance spectrophtometry |
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