| 酸性染料比色法测定虎舌红中总生物碱的含量 |
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| 引用本文： | 凌育赵,杨妙贤. 酸性染料比色法测定虎舌红中总生物碱的含量[J]. 时珍国医国药, 2007, 18(7): 1597-1598 |
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| 作者姓名： | 凌育赵 杨妙贤 |
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| 作者单位： | 仲恺农业技术学院,广东,广州,510225 |
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| 基金项目： | 仲恺农业技术学院科研基金资助项目(No.G3052727) |
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| 摘 要： | 目的建立测定虎舌红总生物碱的含量测定方法。方法采用溶剂萃取酸性染料比色法测定,以去氢飞廉碱(C16H22N4O2)为对照品,溴甲酚绿为酸性染料,用氯仿萃取,检测波长418 nm。结果去氢飞廉碱在0.72~14.02 mg/L范围内与吸光度呈良好线性关系,回归方程为:Y=19.254X-1.6247,r=0.999 5(n=5),平均回收率为101.65%,RSD为0.75%(n=5)。结论所建立方法简便、准确、选择性强。
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| 关 键 词： | 虎舌红总生物碱 酸性染料比色法 去氢飞廉碱 |
| 文章编号： | 1008-0805（2007）07-1597-02 |
| 收稿时间： | 2007-01-04 |
| 修稿时间： | 2007-01-042007-03-08 |
Determination of the Total Alkaloids in Ardisia mamillata by Acid Dye Colorimetry |
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| LING Yu-zhao,YANG Miao-xian. Determination of the Total Alkaloids in Ardisia mamillata by Acid Dye Colorimetry[J]. Lishizhen Medicine and Materia Medica Research, 2007, 18(7): 1597-1598 |
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| Authors: | LING Yu-zhao YANG Miao-xian |
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| Affiliation: | Department of Chemistry and Chemical Engineering, Zhongkai Agriteehnologieal College, Guangzhou 510225, China |
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| Abstract: | Objective To establish a method for quantitative determination of the total alkaloids in the root of Ardisia mamillata.Methods The content was determined by solvent extraction and acid dye colorimetiy,with Acanthoine Ruscope-ine(C16H22N4O2) as a control criterion and bromocresol green as the acid dye,and the wavelength was at 418 nm.Results Acanthoine Ruscope-ine showed a good linear relationship with absorbance when its concentration was within 0.72~14.02 mg/L. The regression equation established was Y=19.254X-1.624 7,r=0.999 5(n=5).The recovery of Acanthoine Ruscope-ine was 101.65%,RSD was 0.75%(n=5).Conclusion This method is easy,accurate and specific. |
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| Keywords: | Ardisia mamillata total Alkaloid Acid Dye Colorimetiy Acanthoine Ruscope-ine |
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