| 自发性高血压大鼠肥厚左室心肌组织微小RNA-1与内向整流钾通道2.1表达的改变及其意义 |
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| 引用本文： | 黄颖,伍伟锋.自发性高血压大鼠肥厚左室心肌组织微小RNA-1与内向整流钾通道2.1表达的改变及其意义[J].高血压杂志,2010(4). |
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| 作者姓名： | 黄颖 伍伟锋 |
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| 作者单位： | 广西医科大学第一附属医院心内科; |
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| 摘 要： | 目的观察自发性高血压大鼠(SHR)肥厚的左室心肌组织微小RNA-1、内向整流钾通道2.1(Kir2.1)表达的变化及其关系,以探讨高血压左心室肥厚(LVH)发生室性心律失常的分子机制。方法取10只17周龄雄性SHR为LVH组,10只8周龄雄性SHR为阳性对照组,10只17周龄雄性WKY大鼠作为空白对照组,通过HE染色、心肌细胞横径测量、实时荧光定量聚合酶链反应(qRT-PCR)、免疫组织化学法及Western blot检测等方法,检测大鼠左室心肌组织病理学改变、微小RNA-1表达、Kir2.1蛋白表达水平的改变。结果①与空白对照组比较,LVH组和阳性对照组的收缩压、舒张压明显升高(分别P<0.01,P<0.05);②与两对照组相比,LVH组的左室质量指数及心肌细胞横径均明显增大(均P<0.05),左室心肌细胞明显肥大,心肌间质增多,伴随着微小RNA-1表达水平明显升高,Kir2.1蛋白表达水平显著降低(P<0.05);③LVH组大鼠左室心肌组织微小RNA-1与Kir2.1蛋白的表达水平呈负相关(r=-0.720,P<0.05)。结论SHR肥厚左室心肌组织微小RNA-1表达上调,并伴随Kir2.1表达下调。
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| 关 键 词： | 微小RNA 自发性高血压大鼠 左心室肥厚 内向整流钾通道2.1 |
The Expression of MicroRNA-1 and Kir2.1 in the Left Ventricular Hypertrophic Tissue of Spontaneously Hypertensive Rats |
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| HUANG Ying,WU Wei-feng.The Expression of MicroRNA-1 and Kir2.1 in the Left Ventricular Hypertrophic Tissue of Spontaneously Hypertensive Rats[J].Chinese Journal of Hypertension,2010(4). |
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| Authors: | HUANG Ying WU Wei-feng |
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| Institution: | HUANG Ying,WU Wei-feng.Department of Cardiology,The First Affiliated Hospital of Guangxi Medical University,Nanning Guangxi 530021,China |
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| Abstract: | Objective To investigate the changes of microRNA-1(miRNA-1) and Kir2.1 in the left ventricular hypertrophic tissue of spontaneously hypertensive rats(SHR,and the correlation between miRNA-1 and Kir2.1).Methods Seventeen-week-old male SHRs with left ventricle hypertrophy(LVH)(n=10),8-week-old male SHRs without LVH(SHR control group n=10) and 17-week-old male Wistar-Kyoto(WKY,n=10) rats were used in this study.The pathological changes,the expression of miRNA-1 and the protein level of Kir2.1 in the left ventr... |
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| Keywords: | MicroRNA Spontaneously hypertensive rats Left ventricular hypertrophy Kir2 1 |
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