Detection of IgA protease from Haemophilus influenzae by immunoblotting |
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| Authors: | T Tsuji R M Alborno M Ehara T Honda T Miwatani |
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| Institution: | (1) Department of Bacteriology and Serology, Research Institute for Microbial Disease, Osaka University, 3-1, Yamadaoka, Suita, 565 Osaka, Japan;(2) Department of Bacterioloa, Institute of Tropical Medicine, Nagasaki University, 12-1, Sakamoto-machi, 852 Nagasaki, Japan |
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| Abstract: | IgA protease produced by various strains of Haemophilus infuenzae can digest serum IgA and yield its fragments which can react with anti-IgA serum. We assayed IgA protease activity by detecting the digests of IgA by SDS-PAGE and immunoblotting. The digests were separated with SDS-PAGE, transferrend to nitrocellulose membranes and detected with anti- ( chain of human IgA, its Fab and its Fc) immunoglobulin conjugated peroxidases.Using this method, we can determine which type of IgA protease is produced by various of H. infuenzae strains. All the 20 strains isolated from respiratory tracts produced IgA protease. |
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| Keywords: | IgA protease Haemophilus influenzae Western blotting |
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