| 分离小鼠腹水中抗HBs/a单克隆抗体杂交瘤细胞制备小鼠腹水 |
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| 引用本文： | 邹汉武,刘琦.分离小鼠腹水中抗HBs/a单克隆抗体杂交瘤细胞制备小鼠腹水[J].华中科技大学学报(医学版),2001,30(1):22-24. |
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| 作者姓名： | 邹汉武 刘琦 |
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| 作者单位： | 武汉生物制品研究所细胞工程室, |
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| 摘 要： | 探讨利用从小鼠腹水中分离获得的单克隆抗体杂交瘤细胞,大量制备小鼠单克隆抗体腹水的方法收集已接种单克隆抗体杂交瘤细胞的小鼠腹水，用淋巴细胞分离液离心分离腹水中的杂交瘤细胞；再将此分离出的杂交瘤细胞，注入其他小鼠腹腔又制得腹水。每只小鼠分离得到的杂交瘤细胞可供5只小鼠腹腔注射,平均每只小鼠产腹水3.97ml。此法可规模制备大量高效价的单克隆抗体腹水。
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| 关 键 词： | 抗体 单克隆 抗HBs/a单克隆抗体 小鼠腹水 |
| 修稿时间： | 2000年10月8日 |
The Preparation of Mice
Ascites with Anti-HBs/a Monoclonal Antibody Hybridoma Cell Separated from Mice Ascites |
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| Zou Hanwu,Liu Qi.The Preparation of Mice
Ascites with Anti-HBs/a Monoclonal Antibody Hybridoma Cell Separated from Mice Ascites[J].Journal of Huazhong University of Science and Technology(Health Sciences),2001,30(1):22-24. |
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| Authors: | Zou Hanwu Liu Qi |
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| Institution: | Zou Hanwu,Liu Qi Cellular Engineering Laboratory,Wuhan Bioproduct Institute,Wuhan 430060 |
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| Abstract: | A large quantity of mice McAb ascites with monoclonal antibody (McAb) hybridoma cells separated from mice ascites was prepared. Ascites of mice inoculated with McAb hybridoma cells were collected, and the McAb hybridoma cells were isolated by centrifugation with lymphocyte separating fluid. McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. McAb hybridoma cells separated from a mouse could be injected into the abdominal cavity of 5 mice. Each mouse could produce 3 97 ml ascites on average. This method could be used to prepare large quantity of high titer mice monoclonal anti HBs/a ascites. |
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| Keywords: | monoclonal antibody anti HBs/a monoclonal antibody mice ascites |
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