氧化石墨烯对大鼠骨髓间充质干细胞生物活性的影响

目的：探讨氧化石墨烯对体外培养的大鼠骨髓间充质干细胞生物学活性的影响。方法采用改良Hummers法制备氧化石墨烯，将不同浓度的氧化石墨烯溶液与大鼠骨髓间充质干细胞共培养，检测其对细胞增殖和形态的影响，相关结果采用SAS V8软件进行统计学分析。结果经表征分析，改良Hummers法可以成功制备出高纯度的氧化石墨烯。在细胞密度较低时，氧化石墨烯对大鼠骨髓间充质细胞的分裂增殖有抑制作用；而在细胞密度较高，达到生长接触抑制的浓度时，氧化石墨烯具有促进细胞死亡，降低活性细胞数量的作用。连续镜下观察后发现，氧化石墨烯对细胞形态有显著影响，可使细胞伸展性下降，折光率降低。这些细胞形态的变化与氧化石墨烯的浓度呈正相关。结论氧化石墨烯可抑制大鼠骨髓间充质干细胞的生物学活性，其作用呈浓度依赖性。

Preparation of Graphene Oxide and Its Effect on Biological Activity of Rat Bone Marrow Mesenchymal Stem Cells

Objective To explore the influences of grapheme oxide （GO） on the biological activity of rat bone marrow mesenchymal stem cells （BMSCs） in vitro. Methods Graphene oxide was prepared by the modified Hummers method. The GO suspension with different concentration was co-cultured with rat BMSCs and then the effects of GO on cell proliferation and morphological change were investigated. The obtained data was statistically analyzed by SAS V8. Results It was shown that highly purified GO could be prepared by the modified Hummers method by atomic force microscope and transmission electron microscope. It was found that GO could inhibit the proliferation of rat BMSCs with low density. When cell density was high, BMSCs reached the concentration of contact inhibition, GO could increase cell death resulting of the reduction of the living cells. Through continuous microscopy observation, it was discovered that GO had obvious impacts on cell morphology. The extensibility and refractivity of rat BMSCs were positively correlated with the concentration of GO. Conclusion GO can inhibit the biological activity of rat BMSCs with dose dependence.