| 壳聚糖作为敏感膜材料的研究 |
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| 引用本文： | 方华丰,周宜开,袁津玮,任恕.壳聚糖作为敏感膜材料的研究[J].华中科技大学学报(医学版),2000,29(3). |
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| 作者姓名： | 方华丰 周宜开 袁津玮 任恕 |
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| 作者单位： | 同济医科大学环境医学研究所,武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目!(No.39990570) |
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| 摘 要： | 以壳聚糖为膜材料制成薄膜 ,运用生物素 -亲和素系统将辣根过氧化物酶 (HRP)固定在膜上 ,固定化过程对 HRP的活性无影响 ,而其稳定性提高 ,运用 L uminol- H2 O2 - HRP化学发光体系 ,对水样中微量 H2 O2 进行检测 ,在 10 - 6～ 10 - 9m ol/ L 范围内线性关系良好 ,回归方程 :Y=2 0 .6 48X- 2 15 .2 39(X:nm ol/ L) ,检测灵敏度为 0 .5 nmol/ L。实验研制的壳聚糖膜可制成生物传感器多功能敏感膜
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| 关 键 词： | 壳聚糖 敏感膜 化学发光 生物传感器 |
Study on Chitosan as Sensing Membrane Materials |
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| Fang Huafeng,Zhou Yikai,Yuan Jinwei Institute of Environmental Medicine,Tongji Medical University,Wuhan.Study on Chitosan as Sensing Membrane Materials[J].Journal of Huazhong University of Science and Technology(Health Sciences),2000,29(3). |
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| Authors: | Fang Huafeng Zhou Yikai Yuan Jinwei Institute of Environmental Medicine Tongji Medical University Wuhan |
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| Institution: | Fang Huafeng,Zhou Yikai,Yuan Jinwei Institute of Environmental Medicine,Tongji Medical University,Wuhan 430030 |
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| Abstract: | Biotin avidin system was used to fix HRP on a thin membrane which was made from chitosan.The trace hydrogen peroxide in water samples was determined by using enhanced chemiluminescence (ECL) detection with Luminol H 2O 2 HRP reaction system. The linear correlation was good at the range of 10 -6 to 10 -9 mol/L. Correlation formulation: Y=20.648X-215.239(X :nmol). The lower detection limit for H 2O 2 was 0.5 nmol. The manufactured HRP chitosan membrane could be used as a sensing membrane of biosensor for detection of H 2O 2 in vivo . |
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| Keywords: | chitosan sensing membrane biosensor chemiluminescene |
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