| Abstract: | Ganoderma applanatum polysaccharide (GAPS) was extracted and purified from Polyporaceae Ganoderma applanatum (Pers.) Pat. It was found that in vitro GAPS (65.2–250 μg/mL) increased the spleen cell proliferation response to ConA; GAPS (500 μg/mL) enhanced IL-2 production of spleen cells stimulated with ConA; GAPS (125–500 μg/mL) augmented the IFNγ production of spleen cells stimulated with ConA. Administering GAPS (20 mg/kg/day s.c.) to normal mice for 10 consecutive days enhanced spleen cell proliferation responses to ConA and the IL-2 and IFNγ production of spleen cells stimulated with 5 μg/mL ConA. It also increased spleen cell primary antibody responses to sheep red blood cells. Administering GAPS (20 mg/kg/day, s.c.) to sarcoma 180 transplanted mice for 15 consecutive days restored the NK activity and the IL-2 and IFNγ production of the spleen cells which were suppressed by the tumour. |
