Lipid Peroxidation of Rat Erythrocyte Membrane Induced by Adriamycin-Fe3+

Adriamycin-Fe³⁺ caused lipid peroxidation of erythrocyte membrane in relation to its concentration. Adriamycin-Fe³⁺ had a high affinity for membrane and the adriamycin-Fe³⁺-binding membranes was also found to cause lipid peroxidation. Under aerobic conditions, adriamycin-Fe³⁺ caused a reduction of cytochrome c and ferrous iron formed spontaneously. Superoxide dismutase (EC 1.15.1.1) (SOD) strongly inhibited the reduction of cytochrome c; however, the enzyme promoted formation of ferrous iron independent of enzymatic action. These results suggest that cytochrome c was reduced by superoxide radical (O₂ ^?) or an adriamycin-iron-O₂ complex such as adriamycin-Fe³⁺–O₂ ^?, but not by adriamycin-Fe²⁺. The ferrous iron chelator bathophenanthroline sulfonate (BPS) completely inhibited oxygen consumption caused by adriamycin-Fe³⁺, indicating that ferrous iron is absolutely required for the lipid peroxidation. SOD and hydroxyl radical scanvengers did not inhibit the lipid peroxidation, indicating that O₂ ^? and hydroxyl radical were not involved in membrane peroxidation. The peroxidation reaction was dramatically inhibited by Tris buffer (2-amino-2-hydroxymethyl-1,3-propanediol). However, hydroxyl radical generation and lipid peroxidation in Tris buffer were not related obviously, indicating that Tris did not act as a hydroxyl radical scavenger. The initial rate of TBARS (thiobarbituric acid reactive substances) formation induced by a mixture of adriamycin-Fe³⁺ and adriamycin-Fe²⁺ was much faster than that indcued by adriamycin-Fe²⁺ or adriamycin-Fe³⁺ alone. These results made it became possible to speculate that the lipid peroxidation might be initiated by an adriamycin-Fe³⁺-oxygen-adriamycin-Fe²⁺ complex.