白术饮片的指纹图谱形状特征与质量标志物(Q-Marker)研究

目的 根据白术Atractylodis Macrocephalae Rhizoma指纹图谱形状特征,建立一种快速精准辨识不同产地与炮制品的方法.方法 建立45批不同产地与炮制法的白术UPLC指纹图谱库,运用总量统计矩及相似度法、正交偏最小二乘-判别分析(orthogonal partial least squares-...

Study on fingerprint shape characteristics and quality markers of Atractylodis Macrocephalae Rhizoma

Objective To establish a rapid and accurate identification method for different processed products and producing areas based on the fingerprint shape characteristics of Baizhu[Atractylodis Macrocephalae Rhizoma (AMR)]. Methods A UPLC fingerprint database of 45 batches of AMR from different producing areas and processing methods was established. The shape characteristics of the fingerprint were analyzed by presidential statistical moment similarity method and orthogonal partial least squares-discriminant analysis (OPLS-DA) method, and the characteristic fingerprint and characteristic peaks were captured. Results There were 16 common peaks in UPLC fingerprints of AMR, bran-processed AMR, soil-processed AMR; The presidential moment similarity between Anhui and Hunan, Anhui and Zhejiang, Hunan and Zhejiang were 0.774-0.982, 0.799-0.969 and 0.788-0.886, respectively. The maximum and minimum of the presidential moment similarity of crude products and bran-processed, crude products and soil-processed, bran-processed and soil-processed AMR from the three areas were 0.990 and 0.774, respectively. Combined with VIP analysis (variable importance for the projection) by OPLS-DA method, 1-3, 6, 16 peaks were the five marker peaks with the largest contribution to discrimination. Peak 6 was identified as atractylenolide II. Conclusion The corresponding substances of these five marker peaks can be identified as the potential quality markers of AMR, which provides theoretical and experimental basis for accurate identification areas, processing method, quality control and evaluation of AMR, and mades active exploration on the quality of TCM fingerprint.