| 致敏树突状细胞介导CTL抗骨髓瘤免疫的实验研究 |
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| 引用本文： | 邓晓辉,梁辉,陈琳军.致敏树突状细胞介导CTL抗骨髓瘤免疫的实验研究[J].上海交通大学学报(医学版),2005,25(5):447-449,482. |
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| 作者姓名： | 邓晓辉 梁辉 陈琳军 |
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| 作者单位： | 上海第二医科大学新华医院血液科,上海,200092;上海第二医科大学新华医院血液科,上海,200092;上海第二医科大学新华医院血液科,上海,200092 |
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| 基金项目： | 上海市科技发展基金(4119024)资助项目. |
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| 摘 要： | 目的比较两种树突状细胞(骨髓瘤致敏DC和未致敏DC)诱导的细胞毒T淋巴细胞抗自身骨髓瘤的细胞免疫：方法用多发性骨髓瘤(MM)患者骨髓CD34^ 细胞诱生DC，将MM患者骨髓瘤冻融物冲击致敏DC。MTT法检测骨髓瘤抗原致敏和未致敏DC诱导的自身T细胞对患者骨髓瘤细胞的杀伤率。结果骨髓瘤冻融物致敏DC诱导的自身T细胞对患者骨髓瘤细胞的杀伤率远大于非致敏DC。结论患者骨髓瘤冻融物致敏的DC能有效诱导自身T细胞抗骨髓瘤免疫。
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| 关 键 词： | 多发性骨髓瘤 树突状细胞 肿瘤冻融物 |
| 文章编号： | 0258-5898(2005)05-0447-04 |
Research of Specific Cytotoxicity T Lymphocyte Response on Myeloma Cells Induced by Myeloma Antigen-Pulsed Dendritic Cells |
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| DENG Xiao-hui,LIANG Hui,CHEN Lin-jun.Research of Specific Cytotoxicity T Lymphocyte Response on Myeloma Cells Induced by Myeloma Antigen-Pulsed Dendritic Cells[J].Journal of Shanghai Jiaotong University:Medical Science,2005,25(5):447-449,482. |
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| Authors: | DENG Xiao-hui LIANG Hui CHEN Lin-jun |
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| Abstract: | Objective To compare cell immunity of cytotoxic T lymphocyte response on myeloma cells induced by dendritic cells with or without myeloma antigen. Methods Dendritic cells (DC) were cultured by CD34 + cells from bone marrow of multiple myeloma (MM) patients. DCs were pulsed with myeloma lysates from MM patients. The killing rates of autologous T lymphocytes induced by DC pulsed with or without myeloma antigen on myeloma cells were measured by MTT method. Results Autologous T lymphocytes induced by DCs pulsed with myeloma lysates had stronger killing effect on myeloma cells than T lymphocytes induced by non myeloma antigen pulsed DC. Conclusion DCs pulsed with myeloma lysates from MM patients can effectively induce autologous T cells anti-myeloma cell immunity. |
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| Keywords: | multiple myeoloma dendritic cells tumor lysate |
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