彩色多普勒超声评价大鼠肝纤维化分期

目的探讨常规二维超声及CDFI评价大鼠肝纤维化分期的价值。方法选取80只健康Wistar大鼠,建立肝纤维化模型,应用二维超声及CDFI测量大鼠门静脉内径及血流速度、脾脏厚度及长度,对测量结果与大鼠肝纤维化病理分期进行相关性分析。结果大鼠门静脉内径在S4与S0、S1、S2、S3之间差异均有统计学意义(P均<0.05),S1与S3、S2与S0的差异有统计学意义(P均<0.05);门静脉血流速度S0与S4的差异有统计学意义(P<0.05)。各期肝纤维化中,脾脏厚度及长度差异均无统计学意义(P均>0.05)。门静脉内径与肝纤维化病理分期呈正相关(r=0.77,P<0.001),门静脉血流速度与肝纤维化分期呈负相关性(r=-0.29,P<0.05)。脾脏厚度及长度与肝纤维化分期无明显相关性。结论常规二维超声及CDFI可通过检测大鼠门静脉内径及血流速度,简便、无创、客观地评价肝纤维化进程。

Color Doppler ultrasonographic staging of rat liver fibrosis

Objective To assess the value of conventional two-dimensional (2D) and color Doppler ultrasonography in the staging of rat liver fibrosis. Methods Eighty healthy Wistar rats were used to establish rat liver fibrosis models, whose portal vein diameter and blood flow velocity, spleen thickness and length were measured by 2D and color Doppler ultrasound, and their correlation with the rat liver fibrosis pathology stage results were analyzed. Results The portal vein diameter of S4 liver fibrosis rats was statistically different from that of S0, S1, S2 and S3 (all P<0.05), and statistical differences were found between S1 and S3, as well as S2 and S0 (both P<0.05). The portal vein blood flow velocity of S4 was significantly different from that of S0(P<0.05). The spleen thickness and length were not significantly different among liver fibrosis stages (all P>0.05). Positive correlation between the portal vein diameter and pathological stage of liver fibrosis was found (r=0.77, P<0.001), while negative correlation between the portal vein blood flow velocity and liver fibrosis stage was observed (r=-0.29, P<0.05). No obvious correlation between the spleen size and liver fibrosis stage was found. Conclusion The portal vein diameter and blood flow velocity measured with conventional 2D and color Doppler ultrasound can be used to evaluate the rat liver fibrosis stage simply, noninvasively and objectively.