2-(3羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖对人胃癌细胞系(SGC-7901)的诱导凋亡作用

目的 观察2-（3-羧基-1-丙酰氨基）-2-脱氧-D-葡萄糖（COADG）体外诱导人胃癌细胞SGC-7901凋亡的作用，探讨其可能的作用机理。方法 采用MTT法，筛选药物作用最佳浓度。用流式细胞仪、琼脂糖凝胶电泳分析诱导凋亡前后的细胞DNA含量，用透射电镜观察凋亡细胞的超微结构。结果 2-（3-羧基-1-丙酰氨基）-2-脱氧-D-葡萄糖能明显抑制胃癌细胞的增长，MTT法显示抑制程度具有时间和剂量效应关系，组间比较表达差异有显著性；流式细胞仪分析可见亚二倍体（Sub—G1）凋亡峰；电镜观察到凋亡小体。结论 2-（3-羧基-1-丙酰氨基）-2-脱氧-D-葡萄糖有诱导人胃癌细胞（SGC-7901）凋亡的作用。

Cell Apoptosis Induced by 2-[ (3-Carboxy-1-Oxopropyl)Amino ]-2-Deoxy-D-Glucose in Human Gastric Cancer SGC-7901 Cells in Vitro

Objective To study the effect of 2-(3-carboxy-1-oxoproyl)amino]-2-deoxy-D-glucose (COADG) on induction of apoptosis in the gastric carcinoma cell line SGC-7901 in vitro.Methods Cell proliferation was examined by MTT assay to chose drug concentration.The changes of the DNA contents were detected by flow cytometer (FCA).The morphological changes of apoptosis cell were observed by electronic microscopy.Results The inhibition of COADG on SGC-7901 was determined by MTT assay in a time-and dose-dependent manner.The apoptosis peak was showed by FCA detection.SGC-7901 cells treated with COADG showed a typical apoptosis changes in morphology.Conclusion COADG can induce SGC-7901 cells apoptosis.