Intervention timing of strontium treatment on estrogen depletion‐induced osteoporosis in rats: Bone microstructure and mechanics |
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| Authors: | Songlin Peng X Sherry Liu Shishu Huang Haobo Pan Wanxin Zhen Guangqian Zhou KDK Luk X Edward Guo W William Lu |
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| Institution: | 1. Department of Spine Surgery, Shenzhen People's Hospital, Jinan University Second College of Medicine, , Shenzhen, China;2. Research Center for Human Tissues and Organs Degeneration, Chinese Academy of Science, , Shenzhen, China;3. Department of Orthopedic Surgery, McKay Orthopaedic Research Laboratory, School of Medicine, University of Pennsylvania, , Philadelphia, Pennsylvania;4. Shenzhen University School of Medicine, , Shenzhen, China;5. Department of Orthopaedics & Traumatology, The University of Hong Kong, , Hong Kong, China;6. Department of Biomedical Engineering, Bone Bioengineering Laboratory, Columbia University, , New York, New York |
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| Abstract: | Purpose To evaluate the effect of intervention timing of Sr treatment on trabecular bone microstructure and mechanics. Methods Ninety female rats were randomly divided into three batches with three groups in each batch. Each group was divided according to the initiation timing of vehicle or strontium compound (SrC), which was at week 0 (early), 4 (mid‐term) and 8 (late) after the ovariectomy, respectively. The treatment lasted for 12 weeks. The trabecular bone biomechanical properties, trabecular bone tissue mechanical properties, trabecular bone microstructure, and bone remodeling were analyzed with mechanical testing, nanoindentation, microCT, and histomorphometry, respectively. The osteoblast and osteoclast phenotypic genes were analyzed with real‐time polymerase chain reaction (PCR). Results Early and mid‐term Sr treatment significantly increased biomechanical properties of trabecular bone, which was associated with increased microarchitecture parameters, increased bone formation parameters and up‐regulation of osteoblast‐related gene expression. Late Sr treatment failed to exert a beneficial effect on any of those parameters. Conclusions The beneficial effect of Sr was dependent on the intervention timing in ovariectomized rats. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:477–484, 2014. |
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| Keywords: | strontium bone microstructure bone remodeling bone mechanics osteoporosis |
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