Skeletal trauma generates systemic BMP2 activation that is temporally related to the mobilization of CD73+ cells |
| |
Authors: | Richard Marsell Brandon Steen Manish V. Bais Douglas P. Mortlock Thomas A. Einhorn Louis C. Gerstenfeld |
| |
Affiliation: | 1. Department of Orthopaedic Surgery, Boston University Medical Center, , Boston, 02118 Massachusetts;2. Department of Molecular Physiology and Biophysics, Center for Human Genetics Research, Vanderbilt University School of Medicine, , Nashville, Tennessee |
| |
Abstract: | The relationship between BMP2 expression and the recruitment of skeletogenic stem cells was assessed following bone marrow reaming. BMP2 expression was examined using transgenic mice in which β‐galactosidase had been inserted into the coding region of BMP2. Stem cell mobilization was analyzed by FACS analysis using CD73, a marker associated with bone marrow stromal stem cells. BMP2 expression was induced in endosteal lining cells, cortical osteocytes and periosteal cells in both the reamed and in contralateral bones. BMP2 mRNA expression in the reamed bone showed an early peak within the first 24 h of reaming followed by a later peak at 7 days, while contralateral bones only showed the 7 days peak of expression. FACS analysis sorting on CD73 positive cells showed a 50% increase of these cells at 3 and 14 days in the marrow of the injured bone and a single peak at 14 days of the marrow cell population of the contralateral bone. A ~20% increase of CD73 positive cells was seen in the peripheral blood 2 days after reaming. These data showed that traumatic bone injury caused a systemic induction of BMP2 expression and that this increase is correlated with the mobilization of CD73 positive cells. © 2013 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 32:17–23, 2014. |
| |
Keywords: | BMP2 reaming marrow stromal cells CD73 CXCR4 |
|
|