Zebrafish brain proteomics reveals central proteins involved in neurodegeneration |
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| Authors: | Mohammed Gebriel Shubhangi Prabhudesai Kai‐Erik Uleberg Eivind Larssen Dominik Piston Anne Hjelle Bjørnstad Simon Geir Møller |
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| Affiliation: | 1. Centre for Organelle Research, University of Stavanger, Stavanger, Norway;2. Department of Biological Sciences, St. John's University, New York, New York;3. International Research Institute of Stavanger IRIS, Stavanger, Norway;4. Department of Pathology, Stavanger University Hospital, Stavanger, Norway;5. The Gade Institute, University of Bergen, Bergen, Norway;6. Norwegian Centre for Movement Disorders, Stavanger University Hospital, Stavanger, Norway |
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| Abstract: | Understanding the complex biology of the brain requires analyzing its structural and functional complexity at the protein level. The large‐scale analysis of the brain proteome, coupled with characterization of central brain proteins, provides insight into fundamental brain processes and processes linked to neurodegenerative diseases. Here we provide a map of the zebrafish brain proteome by using two‐dimensional gel electrophoresis (2DE), followed by the identification of 95 brain proteins using mass spectrometry (LC‐ESI MS/MS). Our data show extensive phosphorylation of brain proteins but less prominent glycosylation. Furthermore, ～51% of the identified proteins are predicted to have one or more ubiquitination sites whereas ～90% are predicted to have one or more SUMOylation sites. Our findings provide a valuable proteome map of the zebrafish brain and associated posttranslational modifications demonstrating that zebrafish proteomic approaches can aid in our understanding of proteins central to important neuronal processes and those associated with neurodegenerative disorders. © 2013 Wiley Periodicals, Inc. |
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| Keywords: | zebrafish brain proteomics neurodegeneration |
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