Enterovirus IgG ELISA using synthetic peptides as antigens |
| |
| Affiliation: | 1. Department of Medical Analytics, Pomeranian Medical University, Al. Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland;2. Department of Laboratory Diagnostics, Independent Public Clinical Hospital No 2, Pomeranian Medical University, Al. Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland;3. Centre for Human Structural and Functional Research, Faculty of Physical Education and Health Promotion, University of Szczecin, ul. Narutowicza 17C, 70-240 Szczecin, Poland;4. Institute of Mathematics, Department of Mathematics and Physics, University of Szczecin, Ul. Wielkopolska 15, 70-451 Szczecin, Poland;5. Department of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University, Al. Powstańców Wielkopolskich 72, 70-204 Szczecin, Poland |
| |
| Abstract: | An indirect IgG enzyme-linked immunosorbent assay (ELISA) based on three different synthetic peptides with amino acid sequences from viral protein VP1 of enteroviruses was explored for the diagnosis of enteroviral infections. In a panel of acute and convalescent sera from 137 patients with culture confirmed infection with one of 17 different enterovirus serotypes, a combination of all three peptides, and the use of two serum dilutions, yielded significant titre rises of enterovirus specific IgG-antibodies in 73% of the patients. This broad-reactive peptide IgG ELISA is useful for serodiagnosis of enteroviral infections. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
