Polymerase chain reaction for the differentiation of Mycobacterium intracellulare and Mycobacterium avium |
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| Institution: | 1. Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina;2. Red Latinoamericana de Vigilancia de la Resistencia a los Antimicrobianos (ReLAVRA);3. Research Career, CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Buenos Aires, Argentina;1. DST/NWU Preclinical Drug Development Platform, Faculty of Health Sciences, North-West University, Potchefstroom, South Africa;2. Statistical Consultation Service, North-West University, Potchefstroom, South Africa;3. Department of Chemical & Biomolecular Engineering, University of Nebraska, Lincoln, USA;1. Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil;2. Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil;3. Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States;4. Laboratório Central, Hospital São Paulo, Division of Laboratory Medicine, Departament of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, Brazil |
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| Abstract: | Polymerase chain reaction (PCR) amplification was performed using DNA purified from 15 mycobacterial type strains and from 21 specimens isolated from patients suspected to have non-tuberculous mycobacterial diseases. Using a primer set of MTB1-MTB2,11 specimens out of 21 were Mycobacterium avium and 8 were M. intracellulare, which were verified by the Gen Probe Rapid Diagnostic System for the M. avium complex (MAC). One of the remaining 2 specimens which did not hybridize with the probe for the MAC was identified as M. kansasii and the other was not specifically identified by the conventional culture method. PCR amplification, using a primer set of TB1-TB3, was also performed for the specific identification of M. tuberculosis complex. |
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