D-dimer test after standardized coagulation: A new assay for global evaluation of the unstimulated fibrinolytic system |
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| Affiliation: | 1. Department of Cardiology, Angiology and Pulmonology, Heidelberg University Hospital, Heidelberg, Germany;2. Goethe University Frankfurt, Frankfurt am Main, Germany;1. Department of Internal Medicine, Division of Dermatology, School of Medicine of Ribeirao Preto, University of Sao Paulo (USP), Av. Bandeirantes, 3900. Monte Alegre, 14049-900, Ribeirao Preto, Sao Paulo, Brazil;2. Graduate Program in Biomedical Sciences, Herminio Ometto University Center (UNIARARAS), Av. Dr. Maximiliano Baruto, 500. Jd. Universitário, 13607-339, Araras, São Paulo, Brazil;3. College of Health Sciences, University of Wisconsin-Milwaukee (UWM), 2400 East Hartford Avenue. Milwaukee, Wisconsin 53211, USA;4. Laboratory of Molecular Pathology, State University of Londrina (UEL), Rod. Celso Garcia Cid, PR-445, Km 380, Londrina, Parana, Brazil;5. Department of Internal Medicine, Division of Clinical Nutrition, School of Medicine of Ribeirao Preto, University of Sao Paulo (USP), Av. Bandeirantes, 3900. Monte Alegre, 14049-900, Ribeirao Preto, Sao Paulo, Brazil;1. Programa de Pós-Graduação em Geociências, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901, Cidade Universitária, Recife, PE, Brazil;2. Laboratório de Paleontologia do Departamento de Ciências Biológicas da Universidade Regional Do Cariri, URCA, Rua Cel. Antônio Luiz, 1161, 63105-000, Pimenta Crato, CE, Brazil;3. Laboratório de Sistemática e Biogeografia (LabSisBio) do Departamento de Zoologia da Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, 20550-013, Maracanã, Rio de Janeiro, RJ, Brazil;4. Museu de Paleontologia Plácido Cidade Nuvens da Universidade Regional do Cariri, URCA, Rua Plácido Cidade Nuvens, 326, 63190-000, Santana do Cariri, CE, Brazil;5. Gondwanan Plants Lab, Universidade Federal de Pernambuco, Centro Acadêmico de Vitória, Rua do Alto Reservatório S/n, Bela Vista, 55608-680, Vitória de Santo Antão, Pernambuco, Brazil;6. Laboratório de Paleontologia e Sistemática da Universidade Federal Rural de Pernambuco, Rua Dom Manuel de Medeiros, S/n, 52171-900, Dois Irmãos, Recife, PE, Brazil |
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| Abstract: | In spite of recently developed analyses for specific components of the fibrinolytic system, a need for global tests still exists. Presently, most global testing is performed either after unpleasent stimulation of the fibrinolytic system, or with time-consuming and imprecise tests.In the present study we have evaluated a D-dimer test (ELISA method) in serum after standardized coagulation in unstimulated individuals, and related the results to those of t-PA and PAI-I activities.28 healthy individuals, 49 patients with coronary heart disease (CHD) and 12 hypertriglyceridemic individuals were tested in the fasting state between 08:00 and 10:00.In the healthy individuals an arbitrary lower reference value of 0.34 ug/ml was established, whereas the median value was 0.86 ug/ml. In the CHD and hypertriglyceridemic groups, the median values were 0.41 ug/ml and 0.18ug/ml, respectively. When the individuals were grouped according to their values to t-PA and PAI-I activities, it emerged that the D-dimer in serum was influenced mainly by PAI-I, but that high values of t-PA determined the global test in some individuals.A good correlation was found between the D-dimer test and both the t-PA (r=0.63) and PAI-I (r=-0.71) activities. We conclude that the presently used D-dimer test in serum is suitable for global testing of the fibrinolytic system. Its clinical relevance has to be evaluated in clinical trials. |
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