Messenger RNA Expression for Growth Factors in Glomeruli from Focal Glomerular Sclerosis |
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| Institution: | 1. Department of Cardiology, Liaocheng People''s Hospital Affiliated to Shandong First Medical University, Liaocheng, Shandong 252000, China;2. Department of Pathology, Liaocheng People''s Hospital, Liaocheng, Shandong 252000, China;3. Stem Cell and Regenerative Medicine Laboratory, Liaocheng People''s Hospital, Liaocheng 252000, PR China;4. Department of Urology, Liaocheng People''s Hospital, Liaocheng 252000, PR China;5. Department of Nursing, Liaocheng Vocational & Technical College, Liaocheng 252000, China;6. Department of Cardiology, Liaocheng People''s Hospital, Shandong University, Jinan, Shandong 250012, China;1. Columbia Center for Human Development, Department of Medicine, Pulmonary Allergy Critical Care, and Department of Genetics and Development, Columbia University Medical Center, New York, NY, 10032, USA;2. Department of Evolutionary Biology, University of Vienna, Vienna, 1030, Austria;3. Institute of Biology, University of Leiden, 2333 BE, Leiden, the Netherlands;1. Department of Immunology, and Key Laboratory of Immunological Environment and Disease, Nanjing Medical University, Nanjing, China;2. Department of Central Laboratory, The Affiliated Huaian No. 1 People''s Hospital, Nanjing Medical University, Huai''an, China;3. Department of Microbiology and Immunology, Jiangsu Health Vocational College, Nanjing, China;4. Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China;5. Key Laboratory of Antibody Technology of Ministry of Health, Nanjing Medical University, Nanjing, China |
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| Abstract: | Focal glomerular sclerosis was induced in rats by chronic injections of puromycin aminonucleoside (PAN) on Days 0, 27, 34, and 41 and by unilateral nephrectomy on Day 22. Rats were sacrificed on Days 0, 8, and 20 (acute phase) and on Days 48, 60, and 80 (sclerotic phase). The percentage of sclerosing glomeruli was 16.6% on Day 48 and increased significantly to 72.8% on Day 80. We examined glomerular mRNA levels for proliferating cell nuclear antigen (PCNA), platelet-derived growth factor (PDGF)-A and B chains, transforming growth factor (TGF)-β, epidermal growth factor (EGF), insulin-like growth factor (IGF)-I, and basic fibroblast growth factor (bFGF) on Days 0, 8, 20, 48, 60, and 80. Although these growth factor mRNA levels showed little change in glomeruli until Day 20, all growth factor mRNA levels increased in glomeruli during the sclerotic phase of PAN nephrosis as glomerular sclerosis progressed. On Day 80, mRNA levels for PCNA, PDGF-A and B chains, TGF-β, EGF, IGF-I, and bFGF increased 12-, 10-, 12-, 15-, 2-, 2-, and 8-fold, respectively, in the glomeruli of PAN-treated rats with marked glomerular sclerosis when compared with control rats. Unilateral nephrectomy without PAN administration did not cause glomerular sclerosis up to Day 80 and mRNA levels for PCNA, PDGF-A and -B chains, TGF-β, EGF, IGF-I, and bFGF in this group were almost the same as those in the normal sham-operated group. These data suggest that changes in growth factor mRNA levels in glomeruli may contribute to the development of PAN-induced glomerular sclerosis. |
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