A xeroderma pigmentosum complementation group A related gene: confirmation using monoclonal antibodies against the cyclobutane dimer and (6-4) photoproduct |
| |
| Affiliation: | 1. Nara Medical University, RI Center, Kashihara, Nara 634, Japan;2. University of Tennessee, Department of Medicine, Memphis, 38163, USA;3. Veterans Affairs Medical Center, Memphis, TN 38104, USA;4. New Jersey Medical School, Department of Microbiology and Molecular Genetics, Newark, NJ 07103, USA;5. Kanazawa University, Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa 920, Japan;6. Vanderbilt University, Department of Biochemistry and Center in Molecular Toxicology, Nashville, TN 37232, USA;1. School of Forensic Medicine, Shanxi Medicine University, Taiyuan, 030001, Shanxi, China;2. The Department of Biochemistry and Molecular Biology, Shanxi Medicine University, Taiyuan, 030001, Shanxi, China;1. People''s Public Security University of China, Beijing 100038, PR China;2. Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China;3. Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Beijing 100038, PR China;4. Key Laboratory of Medical Epigenetics, Tianjin Research Center of Basic Medical Sciences, Tianjin 300070, PR China;1. IPATIMUP (Institute of Molecular Pathology and Immunology of the University of Porto), Porto, Portugal;2. I3 s (Instituto de Investigação e Inovação em Saúde, Universidade do Porto), Porto, Portugal;3. Sección de Genética Forense y Criminalística, Servicio de Laboratorio, Instituto de Medicina Legal y Ciencias Forenses de Valencia, Spain;4. FCUP − Faculty of Sciences of the University of Porto, Portugal;5. Laboratorio Biología-ADN, Unidad Central de Análisis Científicos, Comisaría General Policía Científica, Madrid, Spain;6. Laboratorio de Genética Forense del Instituto de Medicina Legal de Las Palmas, Spain;7. Laboratório de Investigação de Paternidade − NAC-FCFAr − UNESP, Brazil;8. GENES SAS, Medellín, Colombia;9. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Centro, Portugal;10. Instituto de Biologia, Universidad de Antioquia, Medellín, Colombia;11. Molecular Genetics Laboratory, Cruz Vital, Ecuadorian Red Cross, Quito, Ecuador;12. Facultad de Ciencias de la Salud, Universidad de Las Américas, Quito, Ecuador;13. DNA Diagnostic Laboratory (LDD), State University of Rio de Janeiro (UERJ), Rio de Janeiro, Brazil;14. Laboratorio de Genética, Universidad Industrial de Santander (UIS), Bucaramanga, Santander, Colombia;15. Identificación Genética − IMBICE, Instituto Multidisciplinario de Biologia Celular (CONICET-UNLP-CIC). La Plata, Argentina;p. Fac. Cs. Naturales y Museo, Universidad Nacional de La Plata (UNLP), La Plata, Argentina;q. Department of Genetics, Institute Criminalistics in Prague, Prague, Czech Republic;r. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Sul, Portugal;s. Department of Biotechnology, Forensic DNA Lab, University of the Western Cape, Cape Town, South Africa;t. Laboratori de Genètica (Department de Biologia), Universitat de les Illes Balears, Palma de Mallorca, Spain;u. Sección de Genética Forense, Instituto de Ciencias Forenses Luis Concheiro, Facultade de Medicina, Universidade de Santiago de Compostela, A Coruña, Spain;v. Laboratorio de Análisis de ADN, Facultad de Ciencias Médicas – UNCuyo, Mendoza, Argentina;w. Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erndio (Bizkaia), Spain;x. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Germany;y. Andros Day Surgery Clinic, Unilab, Forensic Genetics Laboratory, Palermo, Italy;z. Instituto Nacional de Toxicología y Ciencias Forenses, Delegación de Canarias, Spain;1. Laboratorio de Genética Forense y Genética de Poblaciones, Depto. de Toxicología y Legislación Sanitaria, Fac. de Medicina, UCM, Madrid, Spain;2. Laboratorio Biología Forense, Departamento de Patología y Cirugía, Universidad Miguel Hernández, Elche, Spain;3. Laboratório DNA referencia, Serviço de Imunologia, Hospital de Clínicas de Porto Alegre, Brazil;4. Laboratorio de Genética Molecular, DNA Profiles, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCI-UdeG), Jalisco, Mexico;5. IACA Laboratorios, Bahía Blanca, Argentina;6. Departamento de Biología del Servicio de Criminalística de la Guardia Civil, Madrid, Spain;7. Unidad de Analisis de ADN (COFyBCF), Buenos Aires, Argentina;8. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Norte, Portugal;9. IdentiGEN − Genetic Identification Laboratory and Research Group of Genetic Identification, Institute of Biology, School of Natural and Exact Sciences (FCEN), University of Antioquia, Medellin, Antioquia, Colombia;10. Genética Molecular de Colombia Ldta, Bogotá, Colombia;11. Departamento de Anatomia y Medicina Legal, Laboratorio de periciales Médico Legales de la Universidad de Málaga, Spain;12. Servicio de Huellas Digitales Genéticas, Facultad de Farmacía y Bioquímica, Universidad de Buenos Aires, Argentina;13. Unit of Legal Medicine, University of Cantabria, Santander, Cantabria, Spain;14. Laboratorio Clínico Genetix, S.A., Panama;15. Laboratório de Genética Aplicada, Departamento de Biologia, Universidade de Aveiro, Portugal;P. Laboratorio Genia Geo, Montevideo, Uruguay;Q. INTS, Paris, France;R. Servicios Médicos Yunis Turbay y Cia, Bogotá, Colombia;1. Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain;2. Grupo de Medicina Xenómica (GMX), Faculty of Medicine, University of Santiago de Compostela, Spain;3. Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia |
| |
| Abstract: | Xeroderma pigmentosum complementation group A was partially complemented by a cosmid genomic clone containing a 42-kb human DNA insert selected with a cDNA clone that we obtained through cDNA competition between the repair-proficient and repair-deficient cell line. The relationship between these two clones was confirmed using PCR amplifications. The enhancement in DNA-repair capacity of the transformants was assessed with the monoclonal antibodies specific for cyclobutane dimers and (6−4) photoproducts and partially correct the xeroderma pigmentosum complementation group A defect. Furthermore, the level of the photoproduct-repair capacity is in agreement with the survival enhancement calculated from the D37 values. This gene was mapped to chromosome 8, suggesting that this may represent one of the defective gene(s) in xeroderma pigmentosum complementation group A. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
