| 非小细胞肺癌组织NDRG1表达及其临床意义 |
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| 引用本文： | 范垂锋,林旭勇,刘楠,刘娣,谢成耀,王恩华. 非小细胞肺癌组织NDRG1表达及其临床意义[J]. 中华肿瘤防治杂志, 2011, 18(3) |
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| 作者姓名： | 范垂锋 林旭勇 刘楠 刘娣 谢成耀 王恩华 |
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| 作者单位： | 中国医科大学基础医学院病理学教研室,辽宁,沈阳,110001 |
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| 摘 要： | 目的:分析非小细胞肺癌(NSCLC)组织中N-myc下游调节基因1(NDRG1)的表达及临床意义.方法:免疫组织化学SP和反转录-聚合酶链式反应(RT-PCR)方法分别检测NDRG1在NSCLC组织中的蛋白和mRNA表达.结果:NDRG1的表达定位于细胞质(46/85,54.1%)、细胞核(18/85,21.2%)和细胞膜(9/85,10.6%),总阳性率为61.2% (52/85).NDRG1在癌旁正常肺组织中的表达(22/85,25.9%)低于肺癌组织,P=0.000.NDRG1在淋巴结转移癌组织中的表达(17/40,42.5%)低于原发灶癌(P=0.003),但高于正常肺组织,P=0.002.NDRG1蛋白表达与肿瘤大小呈正相关,肿瘤≥3 cm组的NDRG1蛋白表达(45/59,76.3%)高于肿瘤<3 cm组(7/26,26.9%),P=0.000.NSCLC组织中NDRG1 mRNA水平高于癌旁正常肺组织,P=0.000.NSCLC组织中NDRG1 mRNA表达水平与蛋白表达水平呈正相关,P=0.025.另外,NDRG1阳性组患者的平均生存时间为(35.84±15.07)个月,明显低于NDRG1阴性组患者(63.69±12.16)个月,P=0.002.结论:NSCLC组织中NDRG1的表达相对于癌旁正常肺组织升高,并随肿瘤体积增大而上调,其过表达可能具有提示患者预后不良的意义.
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| 关 键 词： | 癌,非小细胞肺 缺氧 预后 |
Clinical significance of NDRG1 expression in non-small cell lung cancer tissues |
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| FAN Chui-feng,LIN Xu-yong,LIU Nan,LIU Di,XIE Cheng-yao,WANG En-hua. Clinical significance of NDRG1 expression in non-small cell lung cancer tissues[J]. Chinese Journal of Cancer Prevention and Treatment, 2011, 18(3) |
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| Authors: | FAN Chui-feng LIN Xu-yong LIU Nan LIU Di XIE Cheng-yao WANG En-hua |
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| Affiliation: | FAN Chui-feng,LIN Xu-yong,LIU Nan,LIU Di,XIE Cheng-yao,WANG En-hua Department of Pathology,College of Basic Medical Sciences of China Medical University,Shenyang 110001,P.R.China |
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| Abstract: | OBJECTIVE: To investigate the expression and clinical significance of NDRG1 in non-small cell lung cancer(NSCLC) tissues.METHODS: The protein and mRNA expression of NDRG1 in NSCLC tissues were examined using immunohistochemistry(IHC) and RT-PCR respectively.RESULTS: NDRG1 expression was found in cytoplasm(46/85,54.1%),nuclear(18/85,21.2%) and cell membrane(9/85,10.6%) in primary lung cancer tissues with a total positive rate of 61.2%(52/85) which was higher than that in normal lung tissues(22/85,25.9%,P=0.0... |
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| Keywords: | carcinoma non-small cell lung oxygen deficiency prognosis |
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