Survivin蛋白在翼状胬肉组织中的表达及其意义

目的探讨Survivin蛋白在原发性和复发性翼状胬肉组织中的表达及其对细胞增殖和凋亡的影响。方法采用免疫组化法检测40例翼状胬肉（原发性翼状胬肉25例,复发性翼状胬肉15例）和20例正常结膜组织中Survivin蛋白及增殖细胞核抗原（PCNA）的表达,观察原发性翼状胬肉、复发性翼状胬肉、正常结膜组织中细胞增殖指数（PI,PCNA标记指数）的差别及其与Survivin蛋白表达的关系。结果 （1）原发性翼状胬肉和复发性翼状胬肉组织中的Survivin蛋白阳性表达率均明显高于正常结膜组织（均P〈0.01）,而原发性翼状胬肉和复发性翼状胬肉之间的差异无统计学意义（P〉0.05）。（2）原发性翼状胬肉和复发性翼状胬肉组织中PI值均明显高于正常结膜组织（均P〈0.01）,但原发性和复发性翼状胬肉之间的差异无统计学意义（P〉0.05）。（3）Survivin蛋白表达阳性的胬肉组织中PI值较Survivin蛋白表达阴性的胬肉组织中高（P〈0.01）。结论翼状胬肉的发生发展可能与Sur-vivin蛋白的异常表达引起细胞增殖和凋亡失衡有关。

Expression of Survivin Protein in Pterygium and Its Significance

Objective To analyse the expression of Survivin in pterygium and the influence on cell proliferation and apoptosis.Methods Sixty cases were selected in the study,including 40 cases with pterygium（primary pterygium 25 cases,recurrent pterygium 15 cases）,and 20 normal cases as the contrast.All of them were measured for expression levels of Survivin,proliferating cell nuclear antigen（PCNA） in conjunctivas by immunohistochemisty.The difference of index PI（detected by PCNA positive expression） in each group and the relationship of Survivin and PI were studied.Results（1） The levels of Survivin in pterygium group were higher than those in the normal conjunctiva group（P0.01）.But there is no difference between the primary pterygium group and recurrent pterygium group（P0.05）.（2） Labeling index PI of PCNA in the pterygium group,both in primary pterygium group and recurrent pterygium group,were higher than those in the normal conjunctiva group（P0.01）.But there is no difference between the primary pterygium group and recurrent pterygium group（P0.05）.（3）The mean PI in the Survivin-positive pterygium was higher than that in the Survivin-negative pterygium（P0.01）.Conclusions The development of pterygium may be related to over-expression of Survivin,the later play an important role in unbalanced cell proliferation and apoptosis.