| HPLC-ELSD法测定消核片中贝母素甲和贝母素乙的含量 |
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| 引用本文： | 田金苗,孟淑智.HPLC-ELSD法测定消核片中贝母素甲和贝母素乙的含量[J].中国药师,2012(10):1440-1442. |
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| 作者姓名： | 田金苗 孟淑智 |
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| 作者单位： | 辽宁省食品药品检验所 |
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| 摘 要： | 目的：建立消核片中贝母素甲和贝母素乙的含量测定方法。方法：采用高效液相色谱法,色谱柱：Diamonsil C18（200mm×4.6 mm,5μm）,乙腈-水-二乙胺（70∶30∶0.03）为流动相,蒸发光散射检测器。结果：贝母素甲和贝母素乙在1.766～5.520μg（r=0.999 5）和1.280～4.000μg（r=0.999 8）之间线性关系良好,回收率为99.1%和98.6%,RSD为1.8%和2.0%。结论：该方法操作简单,分离效果好,灵敏度高。
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| 关 键 词： | 消核片 贝母素甲 贝母素乙 含量测定 高效液相色谱法 |
| 收稿时间： | 2012/5/12 0:00:00 |
| 修稿时间： | 8/1/2012 12:00:00 AM |
Determination of Peimine and Peiminine in Xiaohe Tablets by HPLC-ELSD |
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| Tian Jinmiao and Meng,Shuzhi.Determination of Peimine and Peiminine in Xiaohe Tablets by HPLC-ELSD[J].China Pharmacist,2012(10):1440-1442. |
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| Authors: | Tian Jinmiao and Meng Shuzhi |
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| Institution: | (Liaoning Institute for Food and Drug Control,Shenyang 110023,China) |
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| Abstract: | Objective: To determine peimine and peiminine in Xiaohe tablets by HPLC.Method: A Diaonsil C18(200 mm×4.6 mm,5 μm)column was used with acetonitrile-water-diethylamine(55∶ 45) as the mobile phase and ELSD as the detector.Result: The linear range was 1.766-5.520 μg(r=0.999 5) and 1.280-4.000 μg(r=0.999 8) with the average recovery of 99.1% and 98.6%,and RSD of 1.8% and 2.0% for peimine and peiminine,respectively.Conclusion: The method is simple and sensitive with good separation efficiency. |
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| Keywords: | Xiaohe tablets Peimine Peiminine Content determination HPLC-ELSD |
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