| 健脾导滞方对人胃癌细胞的作用 |
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| 引用本文： | 闵存云,李庆明,刘和强.健脾导滞方对人胃癌细胞的作用[J].中药材,2004,27(12):935-937. |
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| 作者姓名： | 闵存云 李庆明 刘和强 |
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| 作者单位： | 1. 广东省人民医院中医科,广州,510080
2. 中山大学附属第二医院中医科,广州,510120 |
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| 摘 要： | 目的:观察中药健脾导滞方对胃癌细胞生长、对P53和VEGF及其受体表达的作用.方法:分别以高、中、低等不同剂量的健脾导滞方制剂对Wistar雄性大鼠进行灌胃,制备含药血清,然后分别用不同剂量组的含药血清培养胃癌细胞,以倒置显微镜及流式细胞仪观测各组胃癌细胞的生长状况,并用免疫组化法检测各组胃癌细胞中P53和VEGF及其受体Flt的表达.结果:与对照组比较,用药组胃癌细胞的生长及细胞周期明显改变;P53和VEGF及其受体的表达明显减弱(P＜0.05).结论:健脾导滞方对胃癌细胞的生长,对P53和VEGF及其受体的表达均有抑制作用.
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| 关 键 词： | 健脾导滞方 胃癌细胞 VEGF P53 |
| 修稿时间： | 2004年6月8日 |
The Effect of "Jian Pi Dao Zhi Fang" on Human Gastric Carcinoma Cells |
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| Min Cunyun ,Li Qingming ,Liu Heqiang.The Effect of "Jian Pi Dao Zhi Fang" on Human Gastric Carcinoma Cells[J].Jorunal of Chinese Medicinal Materials,2004,27(12):935-937. |
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| Authors: | Min Cunyun Li Qingming Liu Heqiang |
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| Institution: | Guangdong Provincial Pepople's Hospital, Guangzhou. |
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| Abstract: | OBJECTIVE: To study the effect of Traditional Chinese medicine "Jian Pi Dao Zhi Fang" (JPDZF) with invigorating the spleen and purgative function on the growth of gastric carcinoma cells and the expression of VEGF and P53 in gastric carcinoma cells. METHODS: Gastric carcinoma cells were cultured in the RPMI-1640 media with 10% serum containing JPDZF for 48 hours. The cell cycle were tested with flow cytometer. Expression of P53, VEGF and its receptor Fit were evaluated by immunohistochemistry. RESULT: The cell cycle of gastric carcinoma cells cultured in the media with JPDZF were different from the control. The expression of P53, VEGF, Flt decreased with the dosage increasing of JPDZF. CONCLUSION: Traaditional Chinese medicine JPDZF can inhibit the growth of gastric carcinoma cells and the expression of P53, VEGF, Flt. |
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| Keywords: | Jian Pi Dao Zhi Fang Gastric carcinoma Cells VEGF P53 |
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