Gemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells |
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Authors: | Xin-yu HUANG Hong-cheng WANG Zhou YUAN Ang LI Mei-lan HE Kai-xing Al Qi ZHENG Huan-long QIN |
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Institution: | 1.Department of Surgery, the Sixth People''s Hospital, Shanghai Jiaotong University, Shanghai 200233, China;2.School of Life Science and Technology, Tongji University, Shanghai 200092, China |
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Abstract: | Aim:To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines.Methods:Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine staining, respectively. The expression of Bcl-2, Bax, NF-κB p65 subunit, and IκBα protein was measured using Western blotting.Results:Gemcitabine 0.01?100 μg/mL inhibited cell proliferation and induced apoptosis in both pancreatic cancer BxPC-3 and COLO 357 cells. Gum mastic 40 μg/mL significantly potentiated the antiproliferative and apoptotic effects of gemcitabine 10 μg/mL after 72-h treatment. When cells were treated with gemcitabine in combination with gum mastic, the IκBα level was increased, whereas NF-κB activation was blocked; the expression of Bax protein was substantially increased, but Bcl-2 protein was down-regulated.Conclusion:Gemcitabine combined with gum mastic causes potent apoptosis in pancreatic cancer cells. The combination may be an effective therapeutic strategy for pancreatic cancer. |
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Keywords: | gemcitabine gum mastic pancreatic cancer apoptosis NF-kappaB Bcl-2 Bax |
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