| 非编码RNA在消化系统肿瘤中的作用 |
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| 引用本文： | 苏琦. 非编码RNA在消化系统肿瘤中的作用[J]. 南华大学学报(医学版), 2022, 0(6): 781-784 |
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| 作者姓名： | 苏琦 |
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| 作者单位： | 南华大学衡阳医学院肿瘤研究所 湖南省肿瘤细胞与分子病理学重点实验室,湖南省衡阳市 421001;娄底市中心医院 消化内科,湖南省娄底市 417000;南华大学衡阳医学院肿瘤研究所 湖南省肿瘤细胞与分子病理学重点实验室,湖南省衡阳市 421001;南华大学衡阳医学院附属第二医院,湖南省衡阳市 421001 |
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| 基金项目： | 国家自然科学基金(8197353,81374013,31000629) 作者简介:潘志兵,硕士,主治医师,研究方向为结肠癌发生与防治分子机制,E-mail为675643731@qq.com。通信作者苏琦,二级教授,博士研究生导师,研究方向为肿瘤发生与防治分子机制,E-mail为suqi1945@163.com。 |
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| 摘 要： | 目的探究LIM激酶1(LIMK1)高表达通过LIMK1/cofilin1通路对人结肠癌SW480细胞生物学特性的影响。 方法构建高表达LIMK1基因SW480细胞。 实验分为空载体组、SW480组、LIMK1高表达组(LIMK1/SW480组)。RT-PCR、Western blotting分别检测LIMK1、Rac1、Pak1、p-LIMK1、cofilin1与p-cofilin1表达。MTT、流式细胞术、划痕实验和侵袭实验检测LIMK1高表达对SW480细胞增殖、细胞周期、迁移和侵袭能力的影响。 结果与空载体组和SW480组比较,LIMK1/SW480组LIMK1 mRNA和蛋白表达、细胞增殖活性、穿膜细胞数升高(P＜0.05),G2/M期细胞百分率、瘢痕距离减少(P＜0.05)。LIMK1/SW480组LIMK1、p-LIMK1、p-cofilin1蛋白较空载体组和SW480细胞组升高(P＜0.05)。各组Rac1、Pak1、cofilin1表达差异无统计学意义(P＞0.05)。 结论LIMK1高表达可能通过抑制LIMK1/cofilin1通路促进SW480细胞增殖、迁移、侵袭。
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| 关 键 词： | 人结肠癌SW480细胞 LIMK1 增殖 迁移 侵袭 LIMK1/cofilin1通路 [ |
| 收稿时间： | 2021-12-24 |
| 修稿时间： | 2022-08-21 |
Role of non-coding RNA in digestive system tumors |
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| SU Qi. Role of non-coding RNA in digestive system tumors[J]. Journal of Nanhua University(Medical Edition), 2022, 0(6): 781-784 |
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| Authors: | SU Qi |
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| Affiliation: | Hunan Key Laboratory of Cancer Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China;Department of Gastroenterology, Loudi Central Hospital, Loudi 417000, Hunan, China;Hunan Key Laboratory of Cancer Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China;Department of Pathology, the Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China |
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| Abstract: | The incidence and mortality of digestive system tumors such as colorectal cancer, gastric cancer and liver cancer are among the top 5 tumors in China. Due to the difficulties in early diagnosis, poor efficacy of conventional surgery and chemotherapy, and drug resistance, the 5-year survival rate is low, which seriously endangers people s life and health. Therefore, it is very important to explore the mechanism of invasion and metastasis and therapeutic targets. At present, the role of non-coding RNA in cancer has become a research hotspot. This column summarizes the role of non-coding RNA in colorectal cancer, gastric cancer and liver cancer in combination with the work of relevant experts, hoping to pro-vide a new perspective and potential targets for tumorigenesis and treatment. |
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| Keywords: | tumor of digestive system non coding RNA invasion and metastasis mechanism target |
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