基于网络药理学分析荷丹片与他汀类药物的协同降脂作用

目的 采用网络药理学的方法,筛选荷丹片组方的活性成分和与高脂血症疾病相关的作用靶点,通过生物过程与通路富集预测其作用机制,从而获得荷丹片与他汀类药物协同作用的依据.方法 利用中药系统药理学分析平台(TCMSP,https://tcmspw.com/tcmsp.php)等数据库及文献筛选荷丹片的活性成分及靶点,利用Gee...

Mechansim Analyse of the Synergistic Lipid-lowering Effect of HeDan Tablet and Statins Based on Network Pharmacology

OBJECTIVE Based on the network pharmacology,the active compounds and targets related to hyperlipidemia of HeDan tablet were searched,and the lipid-lowering mechanism were predicted by biological process and pathway enrichment,to provide the theory basis of synergistic lipid-lowering effect of HeDan tablet and statins. METHODS The active constituents and targets were acquired from online databases including TCMSP,and the main disease targets were obtained from OMIM online database. The effective lipid-lowering targets in HeDan tablet were screened,and the Protein-Protein interaction,biological process analysis and pathway enrichment were constructed by Cytoscape and String online database. The lipid lowering mechanism of HeDan tablet was proved and the possibility of synergism between HeDan tablet and statins were explored. RESULTS 163 active compounds of HeDan tablet and 52 targets related to hyperlipidemia were acquired from screening. Protein-Protein interaction network showed that AKT1,ALB,INS,GAPDH and IL6 were the key targets totreat hyperlipidemia. KEGG pathway enrichment showed that adipocytokine signaling pathway,non-alcoholic fatty liver signaling pathway,insulin resistance and glucagon signaling pathway,arachidonic acid metabolism pathway and fatty acid biosynthesis were significantly associated with the treatment of hyperlipidemia,similar to statins. This work proved the synergistic lipid-lowering effect of HeDan tablet and statins. CONCLUSION The study predicted the lipid-lowering mechanism of HeDan tablet based on network pharmacology,reflected the characteristics of multi-component and multi-target of traditional Chinese medicine,provided theoretical basis for the synergistic lipid-lowering effect between HeDan tablet and statin.