肉桂多次ig给药在肾阳虚大鼠体内的积累和代谢分析

目的 考察肉桂多次ig给予肾阳虚大鼠后，鉴定大鼠肾脏、血液、尿液、粪便中原型成分和代谢产物。方法 采用ip氢化可的松方法制备大鼠肾阳虚模型，造模成功后按照生药量1.35g·kg^-1·d^-1ig肉桂提取物，连续15d。末次给药后，收集大鼠血浆、尿液、粪便和肾组织样品，进行UPLC-LTQ-Orbitrap-MS分析。根据色谱保留时间、精确相对分子质量、多级质谱碎片信息结合文献报道对各样本中的成分进行确认，并推测代谢物的代谢途径。结果 从肾阳虚模型大鼠各样本中共鉴定出24个成分，其中3个原型成分，21个代谢产物，包括12个原花青素类、8个木脂素类、2个二萜类、2个有机酸。结论 肉桂成分在肾阳虚大鼠体内代谢广泛，代谢途径主要包括甲基化、硫酸化和葡萄糖醛酸化反应。尿液中检出的化学成分最多，其次为血液、肾组织、粪便。长期给药情况下以原型存在于靶器官和血中成分很少，推测代谢物发挥药效的可能性较大。

Analysis of accumulation and metabolism of Cinnamomum cassia after multiple intragastric administration in kidney-yang deficiency rats

Objective To investigate the archetypal components and metabolites in kidney, blood, urine and feces of kidney-yang deficiency rats after repeated administration with Cinnamomum cassia.Method The rat model of kidney-yang deficiency was established by intraperitoneal injection of cortisol, and the extract of C. cassia was administered at the dosage of 1.35 g·kg^-1·d^-1 for 15 days. Plasma, urine, fecal and renal tissue samples were collected after the last administration of the extract of C. cassia and analyzed by UPLC-LTQ-Orbitrap-MS. Based on the retention time of chromatography, accurate relative molecular mass, and multilevel mass spectrometry fragment information, the components in each sample were identified and the metabolic pathways of metabolites were inferred.Results A total of 24 components (three protypes and 21 metabolites) were identified, including 12 procyanidins, 8 lignans, two diterpenoids and two organic acids.Conclusion Components of C. cassia have a wide metabolism in kidney-yang deficiency rats, and the metabolic pathways mainly include methylation, sulfation and glucuronic acid reaction. Most of the chemicals were found in urine, followed by blood, kidney tissue and feces. In the case of long-term administration, there are few components in the target organs and blood in the prototype, so it is more likely that the metabolites will exert their effects.