溶脲脲原体生物分型方法的建立及应用

目的: 建立检测溶脲脲原体(Uu)生物型别的方法,研究两种Uu生物型(Parvo型和T960型 )感染与人精液质量之间的关系。　方法: 根据Uu两个生物型在 16S~23SrRNA间隔区基因序列,分别设计了Uu种族特异性引物及Uu两种生物型共 3对引物,检测了 949例生殖门诊男性患者的精液常规和精液Uu。　结果: 精液Uu培养阳性且UuPCR检测也呈阳性者 199例 (感染率 21. 1% ),其中Parvo型 136例 ( 136 /199, 68. 3% ),T960型 54例 ( 54 /199,27. 1% ),Parvo型和T960型同时阳性者 9例(9 /199, 4. 5% )。Uu感染T960型组与Parvo组和阴性组相比,精子活动力则明显下降(P<0. 05)。而精子活动率和精子密度无统计学差异。　结论: Uu感染的生物型与人精液质量有关,T960型Uu感染与精子活动力明显相关。

Establishment and Application of the Approach to Detecting Two Biovars of Ureaplsma Urealyticum in Human Semen

Objective: To establish the approach to detecting two biovars of Ureaplasma urealyticum(Uu) in human semen and to investigate the relationship between the two biovars of Uu infection and the quality of human semen. Methods: Based on the 16S-23S rRNA intergenic spacer region, three pairs of primers were designed, the species specific primer and two biovars primers(Parvo primer and T 960 primer). The two biovars of Uu were detected in the semen from 949 men by semen culture and PCR assay. Meanwhile, semen routine analyses were performed. Results: In the 949 subjects, 199 were Uu positive both in Uu liquid culture and PCR assay (199/949, 21.1%), of which 136 (136/199, 68.3%) were Parvo biovar, 54 (54/199, 27.1%) T 960 biovar, and 9 (9/199, 4.5%) both Parvo and T 960 biovars. Compared with the Parvo and the negative groups, human sperm viability was significantly decreased(P< 0.05) in the Uu T 960 infection group. The difference of sperm motility and density had no statistic significance. Conclusion: A significant correlation has been found between Uu T 960 biovar infection and human sperm viability.