曲格列酮对人胃癌细胞生长及转移能力的影响

目的 探讨PPARγ合成配体曲格列酮（TGZ）对胃癌细胞生长、黏附及侵袭转移能力的影响,明确PPARγ配体与胃癌的关系.方法 采用免疫荧光细胞化学法检测PPARγ在胃癌细胞株MGC803中的表达,通过MTT比色法检测不同浓度的TGZ对胃癌细胞增殖活性和体外黏附能力的影响.用体外侵袭系统检测TGZ对MGC803胃癌细胞体外侵袭转移和趋化运动能力的影响.结果 PPARγ在胃癌细胞MGC803中主要表达于细胞核;TGZ抑制胃癌细胞的生长活性,且随着配体药物浓度的升高,胃癌细胞增殖逐渐降低,呈剂量依赖趋势;当TGZ浓度为0.1、1.0及10 μmol/L时,细胞侵袭及运动抑制率分别为8.79%、31.31%、51.42%及28.29%、4.27%、59.27%,与对照组比较差异有统计学意义（P〈0.05）;当TGZ浓度为10μmol/L时,细胞黏附能力为0.32±0.03,与对照（0.52±0.04）相比差异有统计学意义（P〈0.05）.结论 人胃癌细胞MGC803表达功能性PPARγ蛋白;TGZ不同程度抑制MGC803细胞的生长、黏附和侵袭,其作用机制还有待于研究.

Effect of troglitazone on the proliferation and the metastasis in gastric cancer cell

Objective To study the effect of PPARγ ligand troglitazone (TGZ) on invasion and metastasis of gastric cancer cells, and investigate the relationship of PPARγ ligand with gastric cancer.Methods The expression of PPARγ in gastric cancer cell line MGC803 were detected by immunofluorescence cytochemistry method. The effect of different density TGZ on proliferation activity and adhesion of gastric cancer cell were detected by MTT chromatometry. The effect of different ligands on invasion and metastasis of gastric cancer cell MGC803 were detected by invasion system in vitro. Results The expression of PPARγ mainly located in cell nucleus. TGZ inhibited the proliferation of gastric cancer cell, decreased cell adhesion, locomotory capacity and invasion to matrigel, which had time and dose-dependent relationship.When treatment with 0. 1,1.0 and 10μ mol/ L TGZ, inhibition ratio of invasion and metastasis of cell was 8.79% ,31.31% ,51.42% and 28.29% ,4. 27% ,59. 27% respectively, which had statistical significance compared with control group( P <0. 05). When treatment was 10μ mol/L TGZ, cell adhesion was 0. 32 ±0. 03, it was statistically significant higher than that in control group (0. 52 ± 0. 04, P < 0. 05 ). Conclusion Human gastric cancer cell line MGC803 expressed functional PPARγ protein. TGZ inhibited adhesion and invasion of MGC803 cell on ECM at different degree, the effect of combination of two ligands was evident, which mechanism of action needed to be further investigated.