| 四种方法检测粪便中轮状病毒的比较 |
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| 引用本文： | 孟继鸿,张建琼,林陵,王晓明,俞泳华,陶惠娥,汤仕忠. 四种方法检测粪便中轮状病毒的比较[J]. 东南大学学报(医学版), 1992, 0(4) |
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| 作者姓名： | 孟继鸿 张建琼 林陵 王晓明 俞泳华 陶惠娥 汤仕忠 |
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| 作者单位： | 南京铁道医学院微生物学教研室，南京铁道医学院微生物学教研室，南京铁道医学院微生物学教研室，南京铁道医学院附院儿科，南京铁道医学院附院儿科，南京铁道医学院附院儿科，南京铁道医学院附院儿科 |
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| 摘 要： | 用电镜(EM)、聚丙烯酰胺凝胶电泳(PAGE)、酶联免疫吸附试验(ELISA)和协同凝集试验(CoA)等四种方法检测腹泻患儿粪便中轮状病毒,在20份四种方法全检标本中,EM 阳性13份,PAGE16份,ELISA14份,CoA16份。在70份PAGE、ELISA 和 CcA 三种方法检测标本中,PAGE 阳性率为76%,ELISA60%,CoA79%。以 PAGE 为参照,ELISA 和 CoA 两法的敏感性各为75%和91%,特异性各为88%和59%,诊断准确度各为79%和83%。本文对四种方法的优缺点作了评价。
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| 关 键 词： | 显微镜检查 电子 聚丙烯酰胺凝胶电泳 酶连接免疫吸附测定 协同凝集试验 轮状病毒 |
COMPARISON OF FOUR METHODS FOR DETECTION OF ROTAVIRUS IN FECAL SPECIMENS |
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| Meng Jihong Zhang Jianqiong Lin Ling. COMPARISON OF FOUR METHODS FOR DETECTION OF ROTAVIRUS IN FECAL SPECIMENS[J]. Journal of Southeast Univ: Medical Sci Ed, 1992, 0(4) |
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| Authors: | Meng Jihong Zhang Jianqiong Lin Ling |
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| Abstract: | A comparative study of electron microscopy(EM),polyacrylamide gel electrophoresis (PAGE),enzyme-linked immunosorbent assay(ELISA)and co-agglutination test (CoA)for detection of rotavirus(RV)in fecal specimens was carried out.Among 20 specimens tested by all the four methods,13 specimens were positive for RV by EM,16 by PAGE,14 by ELISA and 16 by CoA.Among 70 specimens tested by PAGE, ELISA and CoA,the positive rate was 76%,60%,and 79%,respectively.Compared with PAGE,the sensitivity of ELISA and CoA was 75% and 91%,the specitivity 88% and 59%,the diagnostic accuracy 79% and 83%,respectively.The advantages and disadvantages of the four methods are discussed,too. |
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| Keywords: | microsopy electron polyacrylamide gel electrophoresis enzyme-linked immunosorbent assay co-agglutination test rotaviruses |
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