| 胃癌单抗-RTA结合物的制备及其细胞毒特性研究 |
| |
| 引用本文： | 黎松,张学庸,陈希陶,樊代明,谈立松,潘惠忠,胡继旺,黄利群,顾益群. 胃癌单抗-RTA结合物的制备及其细胞毒特性研究[J]. 现代免疫学, 1988, 0(5) |
| |
| 作者姓名： | 黎松 张学庸 陈希陶 樊代明 谈立松 潘惠忠 胡继旺 黄利群 顾益群 |
| |
| 作者单位： | 第四军医大学西京医院消化病研究室(黎松，张学庸，陈希陶，樊代明)，上海市第一胸部肿瘤防治院生化室(谈立松，潘惠忠，胡继旺，黄利群)，上海市第一胸部肿瘤防治院生化室(顾益群) |
| |
| 摘 要： | 本文报道鼠抗人胃癌单克隆抗体-蓖麻毒素A链结合物的制备及其对肿瘤靶细胞的杀伤作用。抗体与3-(2-吡啶二巯基)丙酸-N-琥珀酰亚胺酯(SPDP)反应引入2-吡啶二硫化物,然后与巯基暴露的A链反应,得到抗体-A链结合物。ELISA结果表明,抗体与毒素A链连接是成功的,结合物中抗体保存较好的与靶细胞结合活性。结合物对靶细胞具有较强的选择性杀伤作用,在1×10~(-9)M水平,对人胃癌细胞KATO Ⅲ蛋白质合成抑制率为71％,而对非靶细胞作用很弱。结果表明,该单抗具有较好毒素A链导向作用。
|
STUDY ON A CONJUGATE OF RICIN A CHAIN AND MURINE MONOCLONAL ANTIBODY AGAINST GASTRIC CANCER CELL AND ITS CANCER CELL SPECIFIC CYTOTOXICITY |
| |
| Abstract: | We have constructed a specific cytotoxic agent by covalently coupling the ricin A chain to monoclonal antibody,MGb2,against gastric cancer cell.MGb2 was modified by SPDP to introduce 3-(2-pyridylthio)propionyl radical and then treated with reduced A chain to give disulfide linked conjugate that retained the original binding specificity of the antibody moiety.The conjugate obtained retained well the activity of antibody and the biological activity of A chain.This conjugate showed selective cytotoxic activity to human gastric cancer cell KATO m in culture.The MTT colometric assay showed that 75% KATOIII cell was killed by adding 10-9 Mol.conjugate to the culture,but the conjugate is not toxic to SL7 which did not react with MGb2.The cytotoxicity of the conjugate was much higher than MGb2 or ricin A chain alone. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
|
