冠元颗粒增强局灶性脑缺血耐受的作用机制研究

目的探讨冠元颗粒对局灶性脑缺血耐受的影响及其作用机制。方法30只SD大鼠随机分为3组,模型组10只予单纯预缺血+缺血(IPC+MCAO),给药组10只予预缺血+冠元颗粒+缺血(IPC+GY+MCAO)处理,假手术组以假手术代替预缺血,余同模型组。利用线栓法建立局灶性脑缺血耐受的动物模型。比较各组神经功能评分、组织病理改变及脑源性神经营养因子(BDNF)表达的变化。结果局灶性缺血预处理能够有效促进大鼠的神经行为功能的恢复,减轻组织学的损伤,增加BDNF表达,其中给药组病理改变最轻,其BDNF水平较模型组有显著性差异(P<0.05)。结论局灶性缺血预处理对随后的脑梗死有明显的保护作用,能够诱导缺血耐受的产生,其可能的机制之一是上调BDNF的表达;冠元颗粒能够进一步诱导其表达的上调,增强其对神经元的保护作用,减轻再次缺血的损伤。

Study of the action mechanisms of Guan-yuan granula on enhancement of focal cerebral ischemia tolerance.

Objective It is to approach the influence and action mechanisms of Guan-yuan granula on focal cerebral ischemia tolerance.Methods 30 SD rats were divided into 3 groups: model group(n=10) with ischemic preconditioning(IPC) ischemia(MCAO),administration group(n=10) with IPC Guan-yuan granula(GY) MCAO and notho-operation group(n=10) with notho-operation MCAO.The model of focal cerebral ischemia tolerance was made by the method of bind.The changes of histological and the expression of brain-derived neurotrophic factor(BDNF) were evaluated.Results Focal cerebral ischemic preconditioning can significantly reduce the following infarct volume and the damage of histology and up-regulate the expression of BDNF.And the effection in the administration group is especially more obvious than the others(P<0.05).Conclusion Focal cerebral ischemic preconditioning can provide brain and induce ischemic tolerance.Up-regulation of the expression of BDNF might play an important mechanism.And Guan-yuan granula can induce the up-regulation of the expression of BDNF,and enhance neurone protection,reduce the second ischemic injure.