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Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein |
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| Authors: | Ke Xu Bo-Jian Zheng Rong Zeng Wei Lu Yong-Ping Lin Li Li Chen Xu Fei Wang Qing-Xi Dong Jia-Rui Wu Bing Sun |
| Affiliation: | a Laboratory of Molecular Virology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai Institutes of Biological Sciences, 225 South Chongqing Road, Shanghai 200025, China b Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China c Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China d Research Center for Proteome Analysis Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China e Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China f E-Institutes of Shanghai University Immunology Division, Shanghai, China |
| Abstract: | Eight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. |
| Keywords: | SARS-CoV ORF9b Virion-associated protein |
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