Poly (I:C) induced immune response in lymphoid tissues involves three sequential waves of type I IFN expression |
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Authors: | Thomas Dé moulins,Marie-Laurence Baron,Nadia Kettaf,Ali Abdallah,Ehssan Sharif-Askari,Rafick-Pierre Sé kaly |
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Affiliation: | a Laboratoire d'Immunologie, Centre de Recherche du CHUM, Hôpital Saint-Luc, Montréal, Québec, Canada H2X 1P1 b INSERM U743, CR-CHUM, Université de Montréal, Montréal, Québec Canada H2X 1P1 c Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada H2X 1P1 d Department of Medicine, Division of Experimental Medicine, McGill University, Montréal, Québec, Canada H2X 1P1 e Département de Microbiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, Québec, Canada H2X 1P1 f Département de Médecine, Université de Montréal, Montréal, Québec, Canada H3T 3J7 |
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Abstract: | An IFN-α heteroduplex-tracking assay (IFN-HTA) was developed to quantify the frequency of expression of the 16 genes coding for related interferon-α (IFN-α) subtypes in mice. In mLN of mice treated with Poly (I:C), we observed the induction of three sequential waves of type I IFN production, instead of two as is commonly described: early IFNs after 1 h (IFN-β), late IFNs after 3 h (mostly IFN-α1, -α2, -α4 and -α5) and “secondary late IFNs” after 6 h (IFN-α6T and -α8/6). The late IFN wave was associated with the upregulation of the interferon regulatory factor (IRF)-7 mRNA and proteins, whereas the secondary late IFN wave was associated with a slight upregulation of IRF-8 mRNA. Type I IFNs produced in the thymus were associated with a distinct IRF mRNA expression pattern. This IFN-HTA strategy can serve as a useful tool to qualify and quantify the expression of various IFN-α subtypes under distinct immune responses and thus provides a first step in evaluating their function. |
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Keywords: | Interferon-α (IFN-α) subtypes Double-stranded RNA Interferon regulatory factor (IRF) IFN-α heteroduplex-tracking assay (IFN-HTA) |
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