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目的探讨PCR方法检测正畸治疗患者口腔中伴放线放线杆菌的临床价值。方法选择2007年1月至2007年6月济南市第四人民医院和济南市口腔医院正畸科患者89例,其中55例正畸治疗至少2个月的患者为实验组,34例未带矫治器的牙周健康者为对照组,记录牙龈炎症指数,分别采集牙周袋最深处或龈沟液标本,应用PCR方法检测伴放线放线杆菌。结果矫治组的伴放线放线杆菌阳性率为52.73%,与对照组(14.71%)间的差异有统计学意义(P<0.05);伴放线放线杆菌的阳性率与牙龈指数之间存在明显的正相关关系(r=0.967)。结论PCR方法可用于检测龈沟液中伴放线放线杆菌,结果准确可靠。

PCR detection of Actinobacillus actinomycetemcomitans in oral specimens from orthodontic patients

Abstract：Objective To establish a PCR for detection of bacteria in oral specimens from orthodontic patients， and analyse the correlation between presence of bacteria and ginival index. Methods Periodotal pocket specimens from fifty-five young patients who wore fixed orthodontic appliances and thirty-four healthy individuals who did not wear orthodontic appliances were collected and the bacteria DNAs were extracted， then polymerase chain reactions were used to amplify the target genes. Meanwhile， anaerobic culture and biochemical event were used to verify PCR results. In addition， the gingival indexes of each individual were recorded. Results The positive rate of PCR assay was coincident with the bacteria culture. The differences between two groups were found to have statistical significance for detection of Aa（P <0.05）. Conclusion The PCR can be used to detect Aa and observe the bacteria changes induced by wearing fixed orthodontic appliances.