麦粒灸对阿霉素所致心肌病大鼠抗氧化应激作用的影响

目的:探讨麦粒灸对阿霉素(DOX)所致心肌病大鼠抗氧化应激作用的影响。方法:SD大鼠随机分为空白组、模型组和麦粒灸组,每组20只。模型组及麦粒灸组腹腔注射DOX复制心肌病模型。麦粒灸组在给药的同时取“关元”“巨阙”穴进行麦粒灸治疗,每日1次,共治疗4周。检测大鼠心率(HR)、左心室收缩压(LVSP)、左心室舒张压(LVDP)、等容收缩期左心室内压最大上升速率(+dp/dtmax)、等容舒张期左心室内压最大下降速率(-dp/dtmax)、左室内压变化时间(t-dp/dtmax)评价心功能;光镜下观察心肌病理组织形态学改变;酶联免疫法测定大鼠血清乳酸脱氢酶(LDH)、磷酸肌酸激酶(CK)及心肌组织中超氧化物歧化酶(SOD)活性及心肌组织中丙二醛(MDA)、血清心肌肌钙蛋白Ⅰ(cTnⅠ)的含量。结果:与空白组比较,模型组HR、LVSP、+dp/dtmax、-dp/dtmax降低,LVDP和t-dp/dtmax升高,差异有统计学意义(P0.05);与模型组比较,麦粒灸组HR、LVSP、+dp/dtmax、-dp/dtmax升高,LVDP和t-dp/dtmax降低,差异有统计学意义(P0.05)。与空白组比较,模型组光镜下呈心肌病样改变,细胞肿胀充血、排列紊乱,白细胞浸润明显;模型组、麦粒灸组大鼠血清LDH、CK活性与cTnⅠ表达水平及心肌MDA含量显著升高(P0.01),心肌SOD活性显著下降(P0.01);与模型组比较,光镜下麦粒灸组心肌细胞轻微肿胀、出血,大鼠血清LDH、CK活性与cTnⅠ表达水平及心肌MDA含量显著降低(P0.01),心肌SOD活性显著升高(P0.01)。结论:麦粒灸可显著减轻DOX引起的心肌细胞损伤,其机制可能与麦粒灸提高SOD活性、降低MDA含量来抑制心肌细胞氧化应激损伤,从而实现对DOX心肌病大鼠的心肌保护作用有关。

Grain-Moxibustion may Protect Myocardium by Reducing Oxidative Stress in Doxorubicin-induced Cardiomyopathy Rats

Objective To observe the effectiveness of grain-moxibustion in resisting oxidative stress in doxorubicin(DOX)-induced cardiomyopathy rats.Methods Sixty Sprague-Dawley(SD)rats were randomly divided into blank control group(blank group),cardiomyopathy model(model)group and grain-moxibustion(moxibustion)group,with 20 rats in each one.The cardiomyopathy model was established by intraperitoneal injection of DOX(0.5mg/100g),once a week for 4weeks.Grainmoxibustion was applied to"Guanyuan"(CV 4)and"Juque"(CV 14)for 5cones,once daily for 4weeks except the Sundays.The rats' heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular diastolic pressure(LVDP),left ventricular pressure maximum rising rate(+dp/dtmax),left ventricular pressure maximum decline rate(-dp/dtmax),left ventricular pressure change time(t-dp/dtmax)were detected to evaluate the cardiac function by using aphysiological signal acquisition-analysis system.The pathological changes of the myocardium were observed by optical microscopy after H.E.staining.The activity of serum lactate dehydrogenase(LDH),creatine kinase(CK)and myocardial superoxide dismutase(SOD),and myocardial malondialdehyde(MDA)and serum cardiac troponinⅠ(cTnⅠ)contents were assayed by enzyme-linked immuno sorbent assay kit(ELISA).Results Following modeling,the HR,LVSP,+dp/dtmax,-dp/dtmax,and myocardial SOD activity were significantly downregulated(P<0.05,P<0.01),while the LVDP,t-dp/dtmax,serum LDH and CK activity and cTnⅠ content,as well as myocardial MDA content were considerably increased in comparison with the blank group(P<0.05,P<0.01).After moxibustion intervention,the decreased HR,LVSP,+dp/dtmax,-dp/dtmax,and myocardial SOD activity levels and the increased LVDP,tdp/dtmax,serum LDH and CK activity and cTnⅠcontent,as well as myocardial MDA content were remarkably suppressed in comparison with the model group(P<0.05,P<0.01).Conclusion Grain-moxibustion can significantly improve the left ventricular function,increase heart rate in cardiomyopathy rats,which may be associated with its effects in reducing myocardial oxidative stress damage by down-regulating the content of MDA and up-regulating SOD activity in the heart.