电针联合天麻多糖对脑缺血大鼠基底外侧杏仁核巢蛋白、脑源性神经营养因子表达的影响

目的:探讨电针联合天麻多糖(PGB)对局灶性脑缺血大鼠基底外侧杏仁核神经干细胞(neural stem cells,NSCs)增殖的影响及其作用机制。方法:40只SD大鼠随机分为正常组、模型组、电针组、PGB组和针药联合组,每组8只。栓塞右侧大脑中动脉制备永久局灶性脑缺血大鼠模型。电针组和针药联合组大鼠给予"百会"、左侧"足三里"穴电针刺激,时间30min,每天1次,连续14d;PGB组和针药联合组大鼠给予PGB 100mg/kg灌胃,每天1次,连续14d。采用Zea-Longa法对各组大鼠进行神经功能评分,免疫组织化学染色方法检测各组大鼠右侧杏仁核区巢蛋白(Nestin)和脑源性神经营养因子(brain derived neurotrophic factor,BDNF)的表达。结果:与模型组比较,各治疗组神经功能评分均降低(P0.05),且针药联合组显著低于电针组及PGB组(P0.05)。与正常组比较,模型组大鼠杏仁核区Nestin和BDNF的表达均增强(P0.05);电针组和PGB组的表达强于模型组(P0.05);针药联合组的表达强于电针组及PGB组(P0.05)。结论:电针与PGB均能够上调脑缺血大鼠杏仁核区Nestin和BDNF的表达,且电针联合PGB的效果更为显著,提示其可能通过促进内源性NSCs的增殖,发挥对缺血区神经元的保护作用。

Effect of Electroacupuncture plus Polysaccharide of Gastrodia elata Blume on Expression of Nestin and Brain Derived Neurotrophic Factor in the Basolateral Amygdala of Focal Cerebral Ischemia Rats

Objective To observe the effect of electroacupuncture(EA)combined with administration of Polysaccharide of Gastrodia elata Blume(PGB)on stem cell proliferation in the basolateral amygdala(BLA)of focal cerebral ischemia(FCI)rats.Methods A total of 40Sprague-Dawley rats were randomly divided into normal control,model,EA,PGB and EA+PGB groups(n=8rats in each group).The FCI model was established by occlusion of the right middle cerebral artery.EA(2Hz,3V)was applied to"Baihui"(GV 20)and the left"Zusanli"(ST 36)for 30 min,once daily for 14 successive days.Rats of the PGB and EA+PGB groups were given with PGB(100mg/kg)by gastrogavage,once daily,for14 successive days.The Zea-Longa's method was used to determine the animals' nerurological dysfunction score on day 1and 14.The expressions of Nestin and brain derived neurotrophic factor(BDNF)of the right BLA were measured by immunohistochemical staining.Results Following modeling,the neurological score,and the numbers of Nestin-and BDNF-immunoreaction(IR)-positive cells were significantly increased in the model group(P<0.05).Compared with the model group,the neurological scores were considerably decreased in the EA,PGB and EA+PGB groups(P<0.05),and the numbers of Nestin-and BDNF-IR positive cells were further significantly increased in the 3treatment groups(P<0.05),and the effect of EA+PGB was notably superior to that of simple EA and simple PGB in down-regulating neurological score and up-regulating Nestin and BDNF expression(P<0.05).Conclusion Both EA and PGB interventions can improve FCI rats' neurological function,which may be related to their effects in up-regulating the expression of Nestin and BDNF to promote the proliferation of neural stem cells in the BLA.