| 高效液相色谱法测定进口芦荟药材中芦荟苷的含量 |
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| 引用本文： | 曹红,刘云.高效液相色谱法测定进口芦荟药材中芦荟苷的含量[J].中国中药杂志,2003,28(4):349-351. |
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| 作者姓名： | 曹红 刘云 |
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| 作者单位： | 解放军总后勤部,卫生部,药品仪器检验所,北京,100071 |
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| 摘 要： | 目的 :建立了测定进口药材芦荟中芦荟苷含量的高效液相色谱法。方法 :采用高效液相色谱法 ,以ZORBAXSB-C18(4.6mm× 2 50mm ,5μm)为色谱柱 ,流动相乙腈 水 (25∶75) ,检测波长 355nm ,流速 1.0mL·min-1 。结果 :芦荟苷在 0.17～5.9μg线性关系良好 ,r=0.9999,芦荟苷平均加样回收率为 98.6% ,RSD 1.32 %(n =6)。结论 :试验结果表明 ,该方法准确、灵敏度高 ,重现性好。
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| 关 键 词： | 芦荟 芦荟苷 高效液相色谱法 |
| 文章编号： | 1001-5302(2003)04-0349-03 |
| 收稿时间： | 2001/12/5 0:00:00 |
| 修稿时间： | 2001年12月5日 |
Determination of Aloin in Aloes by HPLC |
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| CAO Hong;LIU Yun.Determination of Aloin in Aloes by HPLC[J].China Journal of Chinese Materia Medica,2003,28(4):349-351. |
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| Authors: | CAO Hong;LIU Yun |
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| Institution: | Institute for Drug and Instrument Control of PLA, Beijing 100071, China. |
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| Abstract: | OBJECTIVE: To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes. METHOD: The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm. RESULT: The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6). CONCLUSION: The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin. |
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| Keywords: | aloe aloin high performance liquid chromatography |
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