| 原白头翁素衍生物对人肝癌HepG-2细胞的诱导凋亡作用 |
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| 引用本文： | 陈国辉,姜峰玉,孙抒. 原白头翁素衍生物对人肝癌HepG-2细胞的诱导凋亡作用[J]. 延边医学院学报, 2011, 34(1): 31-33 |
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| 作者姓名： | 陈国辉 姜峰玉 孙抒 |
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| 作者单位： | 延边大学基础医学院病理学与法医学教研部,吉林,延吉,133002 |
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| 摘 要： | [目的]观察原白头翁素衍生物对人肝癌HepG-2细胞的诱导凋亡作用.[方法]应用MTT法、HE染色及AO/EB荧光染色方法观察原白头翁素衍生物对人肝癌HepG-2细胞形态的影响.[结果]原白头翁素衍生物对人肝癌HepG-2细胞增殖抑制作用呈质量浓度依赖性和时间依赖性(P<0.05,P<0.01).倒置显微镜下见,给予原白头翁素衍生物5.0 mg/L后早期细胞出现凋亡,晚期细胞发生碎裂坏死;HE染色和AO/EB荧光染色观察见细胞出现典型凋亡形态学变化.[结论]原白头翁素衍生物对人肝癌HepG-2细胞有抑制增殖和诱导凋亡作用.
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| 关 键 词： | 肝肿瘤 白头翁 细胞凋亡 |
Induced apoptosis effects of human hepatoma HepG-2 cells by the isomycin derivant |
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| CHEN Guo-hui,JIANG Feng-yu,SUN Shu. Induced apoptosis effects of human hepatoma HepG-2 cells by the isomycin derivant[J]. Journal of Medical Science Yanbian University, 2011, 34(1): 31-33 |
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| Authors: | CHEN Guo-hui JIANG Feng-yu SUN Shu |
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| Affiliation: | (Department of Pathology and Forensic Medicine,Yanbian University College of Basic Medicine,Yanji 133002,Jilin,China) |
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| Abstract: | OBJECTIVE To observe the morphological changes of the apoptosis and degeneration of human hepatoma HepG-2 cells after treatment with the isomycin derivant.METHODSThe growth inhibition rates and cytotoxic effects of HepG-2 cells were measured with MTT assay.Morphological changes were observed by inverted microscope,HE and AO/EB double staining and fluorescence microscope.RESULTSThe isomycin derivant could significantly inhibit the growth and proliferation of HepG-2 cells in dose-dependent and time-dependent manner.Inverted microscope revealed that the HepG-2 cells showed the apoptosis changes after treated with 5.0mg/L of the isomycin derivant.CONCLUSIONIsomycin dervant could induce the apoptosis of human hepatoma HepG-2 cell line. |
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| Keywords: | liver neoplasms pulsatilia chinensis apoptosis |
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